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首页> 外文期刊>Modern Pathology >HER2 gene amplification occurs frequently in the micropapillary variant of urothelial carcinoma: analysis by dual-color in situ hybridization
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HER2 gene amplification occurs frequently in the micropapillary variant of urothelial carcinoma: analysis by dual-color in situ hybridization

机译：HER2基因扩增经常发生在尿路上皮癌的微乳头变体中：双色原位杂交分析

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Human epidermal growth factor receptor-2 (HER2) is a well-recognized growth-promoting factor in cancer, although its application to urothelial carcinoma has been limited because of a low frequency of gene amplification. We evaluated HER2 protein expression and gene amplification in micropapillary carcinoma, a rare but highly aggressive variant of urothelial carcinoma by dual-color in situ hybridization. Gene amplification was defined by a HER2:CHR17 ratio of ≥2.2; low and high levels of amplification were further defined as in situ hybridization and immunohistochemistry results for evaluation. Overall, 68% (n=13) demonstrated HER2 protein expression of 2+ to 3+ staining. Gene amplification was present in 42% of samples (n=8), with 100% correlation with 2+ and 3+ protein expression. Gene amplification and protein expression were significantly associated (P=0.01). Overall, 53% of samples (n=10) had aneusomy of chromosome 17. Chromosome 17 aneusomy was present in approximately half of the samples evaluated, suggesting inherent genomic instability in this variant of urothelial carcinoma. However, increased HER2:CHR17 ratios demonstrate increased HER2 expression due to amplification in the majority of micropapillary carcinomas. These results suggest that HER2-targeted therapy may be successful on the genomic level in patients with this disease.
机译：人表皮生长因子受体2（HER2）是癌症中公认的生长促进因子，尽管由于基因扩增的频率低，其在尿路上皮癌中的应用受到限制。我们通过双色原位杂交评估了微乳头状癌中HER2蛋白的表达和基因扩增，这是一种罕见的但具有高度侵袭性的尿路上皮癌。基因扩增的定义是HER2：CHR17比率≥2.2;低和高水平的扩增被进一步定义为原位杂交和免疫组化结果进行评估。总体而言，68％（n = 13）表明HER2蛋白表达为2+至3+染色。基因扩增存在于42％的样本中（n = 8），与2+和3+蛋白质表达具有100％的相关性。基因扩增和蛋白质表达显着相关（P = 0.01）。总体而言，有53％的样本（n = 10）具有染色体17的气肿。约有一半的样本中存在17号染色​​体的气肿，这表明该尿路上皮癌变体具有固有的基因组不稳定性。然而，由于大多数微乳头状癌中的扩增，增加的HER2：CHR17比值表明HER2表达增加。这些结果表明，以HER2为靶点的治疗在该疾病患者的基因组水平上可能是成功的。

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