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首页> 外文期刊>Modern Pathology >Expression of Interleukin-6, Interleukin-6 Receptor (gp80), and the Receptor's Signal-Transducing Subunit (gp130) in Human Normal Pituitary Glands and Pituitary Adenomas
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Expression of Interleukin-6, Interleukin-6 Receptor (gp80), and the Receptor's Signal-Transducing Subunit (gp130) in Human Normal Pituitary Glands and Pituitary Adenomas

机译：白细胞介素6，白细胞介素6受体（gp80）和受体信号转导亚基（gp130）在人正常垂体和垂体腺瘤中的表达

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Interleukin-6 (IL-6) is an important cytokine in cell proliferation and differentiation in several organs. It has also been reported that IL-6 plays a role in secretion or release of pituitary hormones in pituitary hormone–secreting cells and pituitary adenomas, but convincing data in situ have not yet been reported. In this study, we examined the participation of IL-6 in the production of pituitary hormones and the differences between human normal pituitary glands and pituitary adenomas by determination of the localization or expression of IL-6, IL-6 receptor (IL-6R, gp80), and the signal-transducing subunit (gp130) of the receptor using immunohistochemical staining and RT-PCR. IL-6 was mainly expressed in ACTH- and FSH/LH-secreting cells in normal pituitary glands, as shown by double staining. gp 80 and gp130 were coexpressed in almost all GH- and PRL-secreting cells and in approximately 30% of FSH/LH-secreting cells. RT-PCR showed that IL-6 mRNA was expressed in only one of all the pituitary adenomas examined, whereas gp 80 and gp 130 mRNAs were detected in all these pituitary adenomas. In conclusion, IL-6 was mainly expressed in ACTH- and FSH/LH-secreting cells, and the receptors were expressed in GH-, PRL- and FSH/LH-secreting cells in human normal pituitary glands. Furthermore, our data emphasized that the mechanism of IL-6 function in human pituitary adenoma cells is distinct from that in normal pituitary cells.
机译：白介素-6（IL-6）是几种器官中细胞增殖和分化的重要细胞因子。也有报道说IL-6在垂体激素分泌细胞和垂体腺瘤中的垂体激素分泌或释放中起作用，但尚未有令人信服的原位数据报道。在这项研究中，我们通过确定IL-6，IL-6受体（IL-6R，IL-6R的定位或表达），研究了IL-6在垂体激素产生中的参与以及人类正常垂体和垂体腺瘤之间的差异。 gp80）和受体的信号转导亚基（gp130），采用免疫组织化学染色和RT-PCR。如双重染色所示，IL-6主要在正常垂体的ACTH和FSH / LH分泌细胞中表达。 gp 80和gp130在几乎所有GH和PRL分泌细胞中以及大约30％的FSH / LH分泌细胞中共表达。 RT-PCR显示IL-6 mRNA仅在所检查的所有垂体腺瘤之一中表达，而在所有这些垂体腺瘤中均检测到gp 80和gp 130 mRNA。总之，IL-6主要在人正常垂体的ACTH和FSH / LH分泌细胞中表达，受体在GH，PRL和FSH / LH分泌细胞中表达。此外，我们的数据强调了人垂体腺瘤细胞中IL-6功能的机制与正常垂体细胞中IL-6功能的机制不同。

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