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首页> 外文期刊>Molecular pain >The analgesic effect of early hyperbaric oxygen treatment in chronic constriction injury rats and its influence on nNOS and iNOS expression and inflammatory factor production
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The analgesic effect of early hyperbaric oxygen treatment in chronic constriction injury rats and its influence on nNOS and iNOS expression and inflammatory factor production

机译：高压氧早期治疗对慢性狭窄大鼠的镇痛作用及其对nNOS和iNOS表达及炎性因子产生的影响

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To observe the analgesic effect of early hyperbaric oxygen (HBO) treatment in chronic constriction injury (CCI) rats, and to analyze the influence of HBO on the expression of neuronal nitric oxide synthase and inducible nitric oxide synthase and on the levels of inflammatory factors. Rats were assigned into three groups randomly: sham, CCI, and HBO groups. The CCI rat model was established, and HBO treatment at 2.5 ATA (60 min) was given one day after surgery, lasting for five consecutive days. The pain behaviors of the rats were observed at predetermined time points, and the activation of astrocytes at dorsal horns as well as the changes of the synaptic ultrastructures were observed. The expressions of inducible nitric oxide synthase and neuronal nitric oxide synthase were detected by Western blot, and the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) were detected by quantitative real-time PCR. Rats in the CCI group developed hyperalgesia when compared with the sham group. Mechanical withdrawal threshold decreased and thermal withdrawal latency shortened in CCI group. Also, astrocytes at the dorsal horn were activated, the synaptic structure was disordered, the expressions of inducible nitric oxide synthase and neuronal nitric oxide synthase were increased significantly, and the release of inflammatory factor (TNF-α and IL-1β) was up-regulated. However, with early initiation of HBO treatment, rats in the HBO group showed significantly alleviated hyperalgesia, increased mechanical withdrawal threshold, and prolonged thermal withdrawal latency. HBO treatment inhibited astrocyte expression and maintained normal synaptic structure. The expressions of inducible nitric oxide synthase and neuronal nitric oxide synthase were decreased in the dorsal horn, and the release of inflammatory factor (TNF-α and IL-1β) was reduced. Early HBO treatment significantly improves hyperalgesia in rats with neuropathic pain. The decreased expressions of inducible nitric oxide synthase and neuronal nitric oxide synthase and reduced levels of inflammatory factors are important mechanisms by which early HBO helps to alleviate neuropathic pain.
机译：观察高压氧（HBO）早期治疗对慢性收缩损伤（CCI）大鼠的镇痛效果，并分析HBO对神经元一氧化氮合酶和诱导型一氧化氮合酶表达以及炎性因子水平的影响。将大鼠随机分为三组：假手术，CCI和HBO组。建立了CCI大鼠模型，并在手术后一天给予2.5 ATA（60分钟）的HBO治疗，连续五天。在预定的时间点观察大鼠的疼痛行为，并观察背角处星形胶质细胞的活化以及突触超微结构的变化。通过蛋白质印迹法检测诱导型一氧化氮合酶和神经元一氧化氮合酶的表达，并通过实时定量PCR检测肿瘤坏死因子-α（TNF-α）和白介素-1β（IL-1β）的水平。与假手术组相比，CCI组的大鼠会出现痛觉过敏。 CCI组机械撤退阈值降低，热撤退潜伏期缩短。此外，背角的星形胶质细胞被激活，突触结构紊乱，诱导型一氧化氮合酶和神经元一氧化氮合酶的表达显着增加，炎性因子（TNF-α和IL-1β）的释放-规范的。然而，随着HBO治疗的提早开始，HBO组的大鼠表现出明显的痛觉过敏减轻，机械停药阈值增加和热停药潜伏期延长。 HBO处理抑制星形胶质细胞表达并维持正常的突触结构。背角诱导型一氧化氮合酶和神经元型一氧化氮合酶的表达降低，炎症因子（TNF-α和IL-1β）的释放减少。早期的HBO治疗可显着改善神经性疼痛大鼠的痛觉过敏。诱导型一氧化氮合酶和神经元一氧化氮合酶的表达降低以及炎症因子水平降低是早期HBO有助于缓解神经性疼痛的重要机制。

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