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首页> 外文期刊>Molecular Plant-Microbe Interactions >The Absence of Eukaryotic Initiation Factor eIF(iso)4E Affects the Systemic Spread of a Tobacco etch virus Isolate in Arabidopsis thaliana
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The Absence of Eukaryotic Initiation Factor eIF(iso)4E Affects the Systemic Spread of a Tobacco etch virus Isolate in Arabidopsis thaliana

机译：真核起始因子eIF（iso）4E的缺乏影响拟南芥中烟草蚀刻病毒分离株的系统传播。

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Translation initiation factor eIF4E exerts an important role during infection of viral species in the family Potyviridae. Particularly, a eIF(iso)4E family member is required for Arabidopsis thaliana susceptibility to Turnip mosaic virus, Lettuce mosaic virus, and Tobacco etch virus (TEV). In addition, a resistance mechanism named restriction of TEV movement (RTM) in A. thaliana controls the systemic spread of TEV in Col-0 ecotype. Here, we describe that TEV-TAMPS, a Mexican isolate, overcomes the RTM resistance mechanism reported for TEV-7DA infection of the Col-0 ecotype but depends on eIF(iso)4E for its systemic spread. To understand at which level eIF(iso)4E participates in A. thaliana TEV-TAMPS infection, the viral RNA replication and translation were measured. The absence or overexpression of eIF(iso)4E did not affect viral translation, and replication was still observed in the absence of eIF(iso)4E. However, the TEV-TAMPS systemic spread was completely abolished in the null mutant. The viral protein genome-linked (VPg) precursor NIa was found in coimmunoprecipitated complexes with both, eIF(iso)4E and eIF4E. However, the viral coat protein (CP) was only present in the eIF(iso)4E complexes. Since both the VPg and the CP proteins are needed for systemic spread, we propose a role of A. thaliana eIF(iso)4E in the movement of TEV-TAMPS within this host.
机译：翻译起始因子eIF4E在波多病毒科的病毒物种感染过程中起着重要作用。特别是，拟南芥对芜菁花叶病毒，生菜花叶病毒和烟草蚀刻病毒（TEV）的敏感性需要eIF（iso）4E家族成员。另外，一种被称为拟南芥中TEV运动限制（RTM）的抗性机制控制了TEV在Col-0生态型中的系统性传播。在这里，我们描述了墨西哥隔离株TEV-TAMPS克服了报道的Col-0生态型TEV-7DA感染的RTM抗性机制，但依赖于eIF（iso）4E的系统传播。为了了解eIF（iso）4E参与拟南芥TEV-TAMPS感染的水平，测量了病毒RNA的复制和翻译。 eIF（iso）4E的缺乏或过表达并不影响病毒翻译，在没有eIF（iso）4E的情况下仍观察到复制。但是，TEV-TAMPS的系统性传播在无效突变体中被完全消除。在与eIF（iso）4E和eIF4E共同免疫沉淀的复合物中发现了病毒蛋白基因组相关（VPg）前体NIa。但是，病毒外壳蛋白（CP）仅存在于eIF（iso）4E复合物中。由于VPg和CP蛋白都是系统扩散所必需的，因此我们提出拟南芥eIF（iso）4E在该宿主内TEV-TAMPS的运动中的作用。

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