...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Cancer >MTA2 promotes gastric cancer cells invasion and is transcriptionally regulated by Sp1
【24h】

MTA2 promotes gastric cancer cells invasion and is transcriptionally regulated by Sp1

机译：MTA2促进胃癌细胞侵袭并受Sp1转录调控

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background MTA2 gene belongs to metastasis associated family, and is highly expressed in some solid tumors, including gastric cancer. Its biological function in gastric cancer is currently undefined. Methods Metastasis-associated tumor gene family 2 (MTA2) and transcription factor specificity protein 1 (Sp1) expression were detected in 127 gastric cancer samples by immunohistochemistry staining. SGC-7901 and AGS gastric cancer cell lines transfected by MTA2 shRNA was used for biological function investigation. Binding and regulation activities of Sp1 on MTA2 promoter were investigated by chromatin immunoprecipitation and luciferase reporter gene. Results The expression rate of MTA2 in gastric cancer tissues was 55.9% (71/127), and its expression was closely related to the depth of tumor invasion, lymph nodes metastasis, and TNM staging. MTA2 knockdown in human SGC-7901 and AGS gastric cancer cells significantly inhibited migration and invasion in vitro , and disrupted structure of cytoskeleton. MTA2 knockdown also attenuated xenografts growth and lung metastasis in nude mice model. MTA2 expression was positively correlated with transcription factor Sp1 in gastric cancer tissues ( r =?0.326, P Conclusions MTA2 knockdown impairs invasion and metastasis of gastric cancer cells, and attenuates xenografts growth in vivo . Sp1 regulates MTA2 expression at transcriptional level.
机译：背景MTA2基因属于转移相关家族，在某些实体瘤（包括胃癌）中高表达。目前尚不清楚其在胃癌中的生物学功能。方法采用免疫组织化学方法检测127例胃癌组织中转移相关肿瘤基因家族2（MTA2）和转录因子特异性蛋白1（Sp1）的表达。将MTA2 shRNA转染的SGC-7901和AGS胃癌细胞系用于生物学功能研究。通过染色质免疫沉淀和荧光素酶报告基因研究Sp1对MTA2启动子的结合和调控活性。结果MTA2在胃癌组织中的表达率为55.9％（71/127），其表达与肿瘤浸润深度，淋巴结转移及TNM分期密切相关。人SGC-7901和AGS胃癌细胞中的MTA2敲低显着抑制了体外迁移和侵袭，并破坏了细胞骨架的结构。 MTA2敲低还减弱了裸鼠模型中异种移植物的生长和肺转移。胃癌组织中MTA2的表达与转录因子Sp1呈正相关（r =？0.326，P结论）MTA2敲低会损害胃癌细胞的侵袭和转移，并减弱体内异种移植物的生长，Sp1在转录水平上调节MTA2的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号