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首页> 外文期刊>Mycobiology >An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
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An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

机译：富含多糖的白J蘑菇银耳的一种改进的总RNA提取方法

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摘要

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus— Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 μg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
机译：描述了一种从果冻蘑菇和双形真菌，尤其是富含多糖的银耳中提取高质量和大量RNA的改进方法。从烟曲霉菌丝体M1332及其亲本单核酵母样细胞Y13和Y32中提取RNA。 A260 / 280和A260 / 230的比率均约为2，RNA完整性数大于8.9。 RNA的产量为108至213μg/ g新鲜wt。还进行了下游分子应用，包括逆转录PCR和定量实时PCR。该方案是可靠的，可以广泛应用于从其他果冻蘑菇或富含多糖的丝状真菌中提取总RNA。

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