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首页> 外文期刊>Molecular Plant-Microbe Interactions >The Type III Secretion Chaperone HpaB Controls the Translocation of Effector and Noneffector Proteins From Xanthomonas campestris pv. vesicatoria
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The Type III Secretion Chaperone HpaB Controls the Translocation of Effector and Noneffector Proteins From Xanthomonas campestris pv. vesicatoria

机译：III型分泌伴侣蛋白HpaB控制来自Xanthomonas campestris pv的效应子和非效应子蛋白的转运。 vesicatoria

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摘要

Pathogenicity of the gram-negative bacterium Xanthomonas campestris pv. vesicatoria depends on a type III secretion (T3S) system, which translocates effector proteins into plant cells. Effector proteins contain N-terminal T3S and translocation signals and interact with the T3S chaperone HpaB, which presumably escorts effectors to the secretion apparatus. The molecular mechanisms underlying the recognition of effectors by the T3S system are not yet understood. In the present study, we analyzed T3S and translocation signals in the type III effectors XopE2 and XopJ from X. campestris pv. vesicatoria. Both effectors contain minimal translocation signals, which are only recognized in the absence of HpaB. Additional N-terminal signals promote translocation of XopE2 and XopJ in the wild-type strain. The results of translocation and interaction studies revealed that the interaction of XopE2 and XopJ with HpaB and a predicted cytoplasmic substrate docking site of the T3S system is not sufficient for translocation. In agreement with this finding, we show that the presence of an artificial HpaB-binding site does not promote translocation of the noneffector XopA in the wild-type strain. Our data, therefore, suggest that the T3S chaperone HpaB not only acts as an escort protein but also controls the recognition of translocation signals.
机译：革兰氏阴性细菌Xanthomonas campestris pv的致病性。 vesicatoria依赖于III型分泌（T3S）系统，该系统可将效应蛋白转运到植物细胞中。效应蛋白包含N端T3S和易位信号，并与T3S伴侣HpaB相互作用，后者可能将效应子护送到分泌装置中。 T3S系统识别效应子的分子机制尚不清楚。在本研究中，我们分析了来自X. campestris pv的III型效应子XopE2和XopJ中的T3S和易位信号。 vesicatoria。两个效应子都包含最小的易位信号，只有在不存在HpaB时才能识别。另外的N端信号促进XopE2和XopJ在野生型菌株中的易位。易位和相互作用研究的结果表明，XopE2和XopJ与HpaB的相互作用以及T3S系统的预计胞质底物对接位点不足以进行易位。与这一发现相一致，我们表明人工HpaB结合位点的存在不会促进野生型菌株中非效应XopA的易位。因此，我们的数据表明，T3S分子伴侣HpaB不仅充当伴游蛋白，而且还控制着对易位信号的识别。

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