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首页> 外文期刊>Nepal Journal of Biotechnology >Molecular Markers for Septoria Leaf Spot (Septoria lycopersicii Speg.) Resistance in Tomato (Solanum lycopersicum L.)
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Molecular Markers for Septoria Leaf Spot (Septoria lycopersicii Speg.) Resistance in Tomato (Solanum lycopersicum L.)

机译：番茄（Solanum lycopersicum L.）的Septoria叶斑病（Septoria lycopersicii Speg。）抗性的分子标记。

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Marker assisted selection (MAS) has not been initiated in tomato (Solanum lycopersicum L.) for septoria leaf spot (SLS) resistance caused by Septoria lycopersici Speg due to lack of molecular markers. We studied the inheritance of SLS resistance and identified molecular markers linked to SLS resistance using bulked segregant analysis (BSA) in a segregating F2 population. Tomato inbred lines, NC 85L-1W (2007), susceptible to SLS and NC 839-2(2007)-1, resistant to SLS were used to develop the segregating population. A total of 250 F2 plants, and 10 plants each of P1, P2 and F1 were grown at the Mountain Horticultural Crops Research and Extension Center (MHCREC), Mills River NC in the summer of 2009. Disease severity was scored using a scale of 0 to 5, where 0 = no disease and 5 = complete development of disease. DNA was extracted from 2-3 week old plants and parental lines were screened with a total of 197 random amplified polymorphic DNA (RAPD) primers, of which 34 were polymorphic. Two DNA bulks, called resistant bulk (RB) and susceptible bulk (SB) were prepared from the F2 individuals. The RB and SB consisted of 8 individuals each with disease scores of 0, and 4.0 or 4.5, respectively. The segregation ratio of resistant and susceptible plants in F2 generation fit the expected Mendelian ratio of 3:1 for a single dominant gene. Five RAPD markers were linked to the SLS disease reaction, of which two were linked to susceptibility and three to the resistance. Subject to verification in independent populations, these markers may be useful for MAS of SLS resistance in tomato.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 40-47
机译：番茄（Solanum lycopersicum L.）由于缺乏分子标记，尚未针对番茄（Solanum lycopersici Speg）引起的葡萄球菌斑斑（SLS）抗性启动标记辅助选择（MAS）。我们研究了SLS抗性的遗传，并在分离的F2群体中使用大体积的分离物分析（BSA）鉴定了与SLS抗性相关的分子标记。对SLS敏感的番茄自交系NC 85L-1W（2007）和对SLS具有抗性的NC 839-2（2007）-1用于培育分离种群。 2009年夏季，在北卡罗来纳州米尔斯河的高山园艺作物研究和推广中心（MHCREC）共种植了250株F2植物，以及P1，P2和F1分别种植了10株植物。使用0进行评分。到5，其中0 =无疾病，而5 =疾病完全发展。从2-3周龄的植物中提取DNA，并用总共197个随机扩增多态性DNA（RAPD）引物筛选亲本，其中34个为多态性。从F2个体中制备了两个DNA主体，称为抗性主体（RB）和易感主体（SB）。 RB和SB由8个个体组成，每个个体的疾病得分分别为0、4.0和4.5。 F2代中抗性植物和易感植物的分离比率符合单个显性基因的预期孟德尔比率3：1。五个RAPD标记物与SLS疾病反应相关，其中两个与药敏性相关，三个与耐药性相关。在独立种群中进行验证后，这些标记物可能对番茄中SLS抗性的MAS有用。尼泊尔生物技术杂志。 2015年12月3，编号1：40-47

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