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首页> 外文期刊>Nepal Journal of Biotechnology >Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation
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Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation

机译：激素对柑桔（Citrus reticulata Blanco）微繁殖的影响

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Tissue culture is the best option to produce disease free seedling of the fruit crop rapidly. Micro-propagation and use of the in-vitro grafting (micro-grafting) is very helpful for production of virus free planting materials in mandarin. Different levels of the in-vitro hormone affect the success of callusing, shooting and plant regeneration in mandarin. Shoot bud, flower bud and in-vitro seedling epicotyl was used as explants to study the hormonal effect on mandarin micro-propagation. Similarly, 10 levels of BAP and IAA combination on MS media for mandarin tissue culture were used. Observation was done for 100 test tubes per treatment combination after 4, 8 and 12 weeks of culture. Data was arc sine transformed for analysis. Shooting from explants was significantly higher (71.72%) on medium level of the BAP (0.5 mg/L) and IAA (0.2 mg/L) using in-vitro seedling stem as explant, however, it was 27.91% for stem bud as explant. Stem bud showed higher level of callusing (6.15%, p<0.001) in mandarin orange. However, flower bud didn’t develop shoot in mandarin tissue culture. Increment of the in-vitro regeneration of the shooting and callusing was observed by the increment of the in-vitro incubation duration in mandarin orange tissue culture.
机译：组织培养是快速生产水果作物无病幼苗的最佳选择。微繁殖和体外嫁接（微嫁接）的使用对普通话无病毒种植材料的生产非常有帮助。不同水平的体外激素会影响普通话中老茧，芽和植物再生的成功。以芽，花芽和离体幼苗的胚轴为外植体，研究荷尔蒙对柑桔微繁的影响。类似地，在普通培养基组织培养的MS培养基上使用了10种水平的BAP和IAA组合。培养4、8和12周后，每种处理组合均观察100个试管。对数据进行反正弦转换以进行分析。以离体幼苗茎为外植体，BAP（0.5 mg / L）和IAA（0.2 mg / L）的中等水平下，外植体的发芽率显着较高，而茎芽为外植体的则为27.91％ 。柑桔中的茎芽显示出较高的愈伤组织水平（6.15％，p <0.001）。然而，在普通话组织培养中，花蕾没有发育成芽。通过柑桔组织培养物中体外孵育持续时间的增加，观察到射击和愈伤组织的体外再生的增加。

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