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首页> 外文期刊>Nepal Journal of Science and Technology >Invitro Screening of Antifungal Activity of Rhizospheric Bacteria and Possible Role of Chitinase in Antifungal Activity
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Invitro Screening of Antifungal Activity of Rhizospheric Bacteria and Possible Role of Chitinase in Antifungal Activity

机译：根际细菌抗真菌活性的体外筛选及几丁质酶在抗真菌活性中的可能作用

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The use of biocontrol agents is becoming an increasingly important alternative to chemical crop protection against weeds, insects and plant diseases in the field of agriculture. The success of biocontrol and yield increase depends on the nature of antagonistic properties and mechanisms of action of the biocontrol agent against the phytopathogens. In this study, 103 macroscopically different bacterial isolates (62 from Kirtipur and 41 from Khumaltar) from 21 different rhizosphere soil samples (11 from Kirtipur and 10 from Khumaltar) were screened for antagonism against five fungal phytopathogens, viz, Fusarium oxysporum, Alternaria solani, Sclerotium rolfsii, Exserohilum turcicum and Phytophthora infestans by dual culture technique on Potato dextrose agar. Out of 18 different active isolates two of them showed the chitinolytic potential and the most active fungal antagonist was identified as Bacillus subtilis on the basis of colonial, morphological, physiological and biochemical characteristics based on Bergey’s Manual of systemic bacteriology. The isolate produced maximum chitinase in colloidal chitin broth at pH7 and temperature 37 o C after four days of inoculation. The corresponding culture filtrate supposed to contain chitinase showed maximum % inhibition of 53.29% with Fusarium oxysporum and no inhibition to Phytophthora infestans in agar well diffusion assay. Furthermore, chitinase was best fractionated at 40% ammonium sulphate salt fractionation which has almost similar inhibition potential as the crude culture filtrate. The 40% salt fraction of the enzyme showed the maximum chitinolytic potential at pH8 and temperature 40 o C. Among the phytopathogens tested, sensitivity of Bacillus subtilis to fungi containing chitin on their cell wall demonstrates the possible role of chitinase in the antifungal activity. DOI: http://dx.doi.org/10.3126jst.v12i0.6517 Nepal Journal of Science and Technology 12 (2011) 304-311
机译：在农业领域，生物防治剂的使用已成为化学作物保护杂草，昆虫和植物病害的重要替代方法。生物防治和产量增加的成功取决于拮抗特性的性质以及生物防治剂对抗植物病原体的作用机理。在这项研究中，针对21种不同的根际土壤样品（分别来自Kirtipur的11种和来自Khumaltar的10种）的103种宏观上不同的细菌分离株（来自Kirtipur的62株和来自Khumaltar的41株）筛选了对五种真菌性植物病原菌的拮抗作用，即真菌，尖孢镰刀菌，链霉菌，通过双重培养技术在马铃薯葡萄糖琼脂上培养了菌核病菌（Sclerotium rolfsii），鹅膏菌（Exserohilum turcicum）和疫霉菌（Phytophthora infestans）。在18种不同的活性分离物中，有2种表现出几丁质分解的潜力，根据伯吉的《系统细菌学手册》，根据菌落，形态，生理和生化特征，最活跃的真菌拮抗剂被鉴定为枯草芽孢杆菌。接种四天后，分离物在胶体甲壳质肉汤中于pH7和温度37 o C时产生最大的几丁质酶。假定含有几丁质酶的相应培养物滤液在尖孢镰刀菌扩散试验中显示出用尖孢镰刀菌最大抑制率为53.29％，而对疫霉疫霉没有抑制作用。此外，几丁质酶最好在40％硫酸铵盐分级中进行分级，其抑制潜力与粗培养滤液几乎相似。该酶的40％盐分在pH8和40 o C的温度下显示最大的几丁质分解能力。在所测试的植物病原体中，枯草芽孢杆菌对细胞壁上含有几丁质真菌的敏感性表明，几丁质酶可能具有抗真菌活性。 DOI：http://dx.doi.org/10.3126jst.v12i0.6517尼泊尔科学技术杂志12（2011）304-311

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