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Pollen Germination in Different Genotypes of Gladiolus

机译:唐Gla蒲不同基因型的花粉萌发

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Fresh pollen of 16 promising genotypes of gladiolus was tested for their pollen viability. Modified cellophane method was employed to assess the pollen viability. Pollen germination media consisted of 15% sucrose supplemented with 300 ppm calcium nitrate, 200 ppm magnesium sulphate, 100 ppm potassium nitrate and 100 ppm boric acid. Highly significant variations were observed for percentage of pollen germination, non-germinated pollen and sterile pollen. The highest pollen germination (76.41%) was in genotype Hybrid selection 88-10-22, and did not differ significantly from Gladiolus callianthus (75.41%), Sapna (75.10 %), Hybrid selection 86-32-11, (73.28 %), Kum Kum (69.41%), Poonam (69.22 %), Hybrid selection 87-22-1 (67.87 %), Hybrid selection 87-1-1 (67.61 %), Psittacinus hybrid (64.64%) and Darshan (63.97%). The lowest non- germinated pollen (10.47%) was in genotype Gladiolus callianthus , and insignificantly followed by Hybrid selection 88-10-22 with 18.77 % and Hybrid selection 87-22-1 with 18.95 %. The lowest percentage of sterile pollen was noticed in genotype Sapna (2.82%) followed by Poonam with 4.00 % Hybrid selection 88-10-22 with 4.82% and Hybrid selection 82-11-27 with 5.22%. Key words: Gladiolus; germination; pollen DOI: 10.3126jst.v11i0.4089 Nepal Journal of Science and Technology 11 (2010) 47-50
机译:测试了16种有前景基因唐promising蒲的新鲜花粉的花粉生存力。采用改进的玻璃纸方法评估花粉的活力。花粉发芽培养基由15%的蔗糖,300 ppm的硝酸钙,200 ppm的硫酸镁,100 ppm的硝酸钾和100 ppm的硼酸组成。花粉发芽,未发芽花粉和不育花粉的百分比观察到非常显着的变化。最高的花粉发芽率(76.41%)在基因型杂交选择88-10-22中,与唐Gla蒲(75.41%),Sapna(75.10%),杂交选择86-32-11(73.28%)没有显着差异。 ,Kum Kum(69.41%),Poonam(69.22%),杂种选择87-22-1(67.87%),杂种选择87-1-1(67.61%),Psittacinus杂种(64.64%)和Darshan(63.97%) 。最低的未发芽花粉(10.47%)是基因型唐Gla蒲,其次是杂种选择88-10-22,占18.77%,杂种选择87-22-1,占18.95%。基因型Sapna(2.82%)中观察到的无菌花粉百分比最低,其次是Poonam(4.00%),杂交选择88-10-22(4.82%)和杂交选择82-11-27(5.22%)。关键字:唐Gla蒲;发芽;花粉DOI:10.3126 / njst.v11i0.4089尼泊尔科学和技术杂志11(2010)47-50

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