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首页> 外文期刊>Nematropica >METHODOLOGY FOR SCREENING FOR RESISTANCE TO BELONOLAIMUS LONGICAUDATUS IN TURFGRASS
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METHODOLOGY FOR SCREENING FOR RESISTANCE TO BELONOLAIMUS LONGICAUDATUS IN TURFGRASS

机译：沙门氏菌对沙门氏菌耐药性的筛查方法

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Schwartz, B. M., K. E. Kenworthy, W. T. Crow, J. A. Ferrell, G. L. Miller, K. H. Quesenberry. 2008. Methodology for screening Belonolaimus longicaudatus in a turfgrass breeding program. Nematropica 38:163-175. Efforts to screen large numbers of turfgrass genotypes would likely result in the discovery of genes associated with resistance or tolerance to plant-parasitic nematodes. The identification of germplasm with these characteristics will become increasingly important as stringent regulations regarding the use of soil fumigants and nematicides are put into place. Therefore, a glasshouse study was initiated to investigate plant-parasitic nematode evaluation methods on 'TifEagle' hybrid bermudagrass to identify a high throughput, accurate and repeatable greenhouse screen useful to turfgrass breeding programs during sequential trials in 2007. Three establishment methods, classified as: i) conetainers grown in for 45 days (45-d conetainers), ii) conetainers grown in for 90 days (90-d conetainers), and iii) clay pots grown in for 90 days (90-d clay pots) before inoculation with sting nematodes, respectively, were assessed. Two inoculation rates, 50 and 100 mixed life stages of Belonolaimus longicaudatus/100 cm3 of soil, were compared to an uninoculated control within each establishment method. Total dry root weights were 38% and 28% larger for uninoculated treatments when compared to an average of the two sting nematode inoculated treatments in the 45-d conetainers and 90-d conetainers, respectively. Root weights were not significantly reduced by sting nematode pressure in the 90-d clay pots. Total root lengths of uninoculated treatments were 57%, 55%, and 31% greater than an average of the two inoculated treatments in the 45-d conetainers, 90-d conetainers, and 90-d clay pots, respectively. Results were generally more variable for treatments grown in 90-d clay pots and some root length characteristics were not consistent between trials in the 90-d conetainers. Quantifying root damage using 45-d conetainers inoculated with 50 sting nematodes provided reproducible results characteristic of those reported in other greenhouse and field evaluations.
机译：Schwartz，B。M.，K。E. Kenworthy，W。T. Crow，J。A. Ferrell，G。L. Miller，K。H. Quesenberry。 2008。在草皮草育种计划中筛选Belonolaimus longicaudatus的方法。 Nematropica 38：163-175。筛选大量草皮草基因型的努力可能会导致发现与对植物寄生线虫的抗性或耐受性相关的基因。随着有关土壤熏蒸剂和杀线虫剂使用的严格规定的出台，具有这些特征的种质的鉴定将变得越来越重要。因此，开始进行温室研究，以研究“ TifEagle”杂种百慕大草上的植物寄生线虫评估方法，以鉴定出高通量，准确且可重复的温室筛查方法，该方法可用于2007年连续试验期间的草皮草育种计划。三种建立方法，分类为： i）接种45天的锥形容器（45-d锥形容器），ii）接种90天的锥形容器（90-d锥形容器），和iii）接种90天之前的锥形罐（90-d锥形容器）分别对ing线虫进行了评估。在每种建立方法中，将两种接种率，即长白带贝母/ 100 cm3的50和100混合生命期与未接种对照进行了比较。与分别在45 d和90 d锥形容器中接种两种st线虫的处理平均值相比，未接种的干根总重分别大38％和28％。在90天的陶罐中，刺线虫压力不会显着降低根重。未接种处理的总根长分别比45 d锥形容器，90 d锥形容器和90 d粘土罐中两次接种处理的平均值分别大57％，55％和31％。对于在90天粘土锅中生长的处理，结果通常更具可变性，并且在90天圆锥形容器的试验之间，某些根部长度特征不一致。使用接种了50根线虫的45 d锥形容器对根部损害进行定量，可提供其他温室和田间评估中报道的可再现结果。

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