首页> 外文期刊>Nematropica >HISTOPATOLOGíA EN CULTIVARES DE FRIJOL (PHASEOLUS VULGARIS L.) NO HOSPEDANTES DE NACOBBUS ABERRANS (THORNE, 1935) THORNE Y ALLEN, 1944
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HISTOPATOLOGíA EN CULTIVARES DE FRIJOL (PHASEOLUS VULGARIS L.) NO HOSPEDANTES DE NACOBBUS ABERRANS (THORNE, 1935) THORNE Y ALLEN, 1944

机译:豆类栽培种(菜豆)的组织病理学未出现纳博布·艾伯伦(Thorn,1935年)和Thornne and Allen,1944年

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Martínez-Fuentes, R., A. Tovar-Soto, R. Torres-Coronel, y A. E. Brechú-Franco. 2009. Histopathology of bean (Phaseolus vulgaris L.) cultivars non-host to Nacobbus aberrans (Thorne, 1935) Thorne y Allen, 1944. Nematropica 39:311-317. We describe the histopathological changes caused by a Nacobbus aberrans population from Chapingo, Mexico on bean cultivars Negro Querétaro and Canario, which are non-hosts to this population. The plants were inoculated with 15,000 eggs and evaluated 15, 45, and 90 days after inoculation (dai). For each date, the roots were embedded in Tissue Teck? O.C.T. Compound? and 10 Um-thick sections were cut with a Microm HM505N? cryostat at -30°C and then stained with basic fuschsine-fast green. At 15 dai on both cultivars, nematodes were trapped in lignified cavities surrounded by hypertrophied cells. The nematodes present were third (J3) and fourth stage juveniles (J4), identified by morphological characters, and remained in these stages until 90 dai. Genetic variability of the nematode populations and of the host cultivars is discussed as related to the diverse histopathological reaction responses on bean.
机译:Martínez-Fuentes,R.,A。Tovar-Soto,R。Torres-Coronel,和A.E.Brechú-Franco。 2009。非寄主于Aberrans aberrans的豆(菜豆)的组织病理学(Thorne,1935)Thorne y Allen,1944。Nematropica39:311-317。我们描述了由墨西哥Chapingo的一个豆品种NegroQuerétaro和Canario上的Nacobbus aberrans种群引起的组织病理学变化,这些品种不是该种群的寄主。用15,000个卵接种植物,并在接种后第15、45和90天(dai)进行评估。对于每个日期,其根都嵌入到Teck组织中? O.C.T.复合?用Microm HM505N?切割10微米厚的切片。低温恒温器,温度为-30°C,然后用碱性品夫松耐牢绿色染色。在两个品种的第15天时,线虫都被困在被肥大细胞包围的木质化空腔中。存在的线虫是第三阶段(J3)和第四阶段的幼虫(J4),通过形态学特征鉴定,并一直保留在这些阶段直到90天。讨论了线虫种群和寄主品种的遗传变异性,其与豆类的各种组织病理学反应有关。

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