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MOLECULAR, MORPHOLOGICAL AND THERMAL CHARACTERS OF 19 PRATYLENCHUS SPP. AND RELATIVES USING THE D3 SEGMENT OF THE NUCLEAR LSU rRNA GENE

机译:19种小菜蛾的分子,形态和热学性质。核LSU rRNA基因的D3段及其相关性

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Gene sequences are provided for the D3 segment of the large subunit rRNA gene in Pratylenchus agilis, P . hexincisus, P . teres, and P . zeae. They were aligned with the closest comparable previously published molecular sequences and evaluated with parsimony, distance and maximum-likelihood methods. Different outgroups and more taxa in this study compared to a previous D3 tree resulted in improved phylogenetic resolution. Congruence of trees with thermal, vulval and lip characters was evaluated. A tropical clade of Pratylenchus with 2 lip annules was seen in all trees. Maximum-Parsimony and Quartet-Puzzling Maximum-Likelihood trees, with ambiguously-alignable positions excluded and Radopholus similis as an outgroup, had topologies congruent with species possessing 2, 3 or 4 lip annules. An updated sequence for Pratylenchus hexincisus indicated it was an outgroup of P . penetrans, P . arlingtoni, P . fallax and P . convallariae . Pratylenchus zeae was related to P . neglectus in a Neighbor-Joining tree, but was equivocal in others. The relatives of P . teres were P . neglectus and Hirschmanniella belli rather than morphometrically similar P . crenatus . The P . agilis sequence is more closely related to the nearly identical sequences of P . pseudocoffeae and P . brachyurus, than to that of P . scribneri, which is a species closely related morphologically.
机译:提供了在Prattylenchus agilis,P中的大亚基rRNA基因的D3区段的基因序列。 hexincisus,P。 teres和P。玉米它们与最接近的可比较的先前公开的分子序列比对,并用简约,距离和最大似然法进行评估。与先前的D3树相比,本研究中的不同群体和更多的分类单元导致系统发育分辨率的提高。评估了具有热,外阴和嘴唇特征的树木的一致性。在所有树木中都看到了一个带有两个唇环的Pratylenchus热带进化枝。最大简约和四重困惑的最大似然树,排除了歧义对齐的位置,而Radopholus similis作为一个整体,其拓扑与具有2、3或4个唇瓣环的物种一致。更新的Pratylenchus hexincisus序列表明它是P的一个外群。 penetrans,P。阿灵顿尼谬误和P。铃兰。斑节菜与P有关。在邻居加入树中被忽视,但在其他树上则模棱两可。 P的亲戚。千人为P。 neglectus和Hirschmanniella belli而不是形态相似的P。克雷纳托斯。 P。敏捷序列与P的几乎相同的序列密切相关。伪咖啡和P。 brachyurus,而不是P。 scribneri,这是一个形态密切相关的物种。

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