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首页> 外文期刊>Neuro-signals >Effects of Staurosporine on Cell Morphology, Expression of the Proenkephalin Gene and the Secretion of [Met5]-Enkephalin in Bovine Adrenal Medullary Chromaffin Cells
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Effects of Staurosporine on Cell Morphology, Expression of the Proenkephalin Gene and the Secretion of [Met5]-Enkephalin in Bovine Adrenal Medullary Chromaffin Cells

机译：星形孢菌素对牛肾上腺髓质嗜铬细胞细胞形态，原脑啡肽基因表达及[Met5]-脑啡肽分泌的影响

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>Treatment of bovine adrenal medullary chromaffin (BAMC) cells with staurosporine for 24 h caused the cells to elongate and flatten, and induced the formation of neurite-like outgrowth from BAMC cells; superficially the cells resembled those treated with the protein kinase C (PKC) agonist phorbol myristate acetate (PMA, 10-7M). The intracellular concentration of [Met5]-enkephalin (ME) was significantly increased in staurosporine-treated cells whereas the secretion of ME into the medium was significantly less than that from control cells. In addition, pretreatment of cells with staurosporine effectively inhibited the long-term stimulatory effects of other secretagogues on ME secretion. Furthermore, a 24 h exposure to staurosporine greatly increased the levels of both proenkephalin A (proENK) and its messenger RNA (mRNA). Both staurosporine and PMA increased AP-1 DNA binding activity to a similar extent. In contrast to the results with staurosporine, the structurally similar compound, K252a (10-8M) did not show these effects. Moreover, other PKC inhibitors, H7 (10-5M) and sphingosine (3.6 x 10-5M), did not duplicate those effects, suggesting that these long-term effects of staurosporine are independent of PKC inhibition. Staurosporine acts at the ATP binding site on many other kinases, but it is presently unclear whether the observed effects on cell morphology, proENK mRNA induction and ME secretion result from inhibition of only a single particular kinase. However, the uncoupling of proENK mRNA induction from ME sermon in these cells is unique to staurosporine and, therefore, this compound may be useful for further studies on secretion-transcription coupling.
机译：>用星形孢菌素处理牛肾上腺髓质嗜铬细胞（BAMC）24小时，使细胞伸长和变平，并诱导BAMC细胞形成神经突状生长。从表面上看，这些细胞类似于用蛋白激酶C（PKC）激动剂佛波醇肉豆蔻酸酯乙酸（PMA，10 -7 M）处理的细胞。 在星形孢菌素处理的细胞中，[Met 5 ]-脑啡肽（ME）的细胞内浓度显着增加，而ME向培养基中的分泌却明显少于对照细胞。此外，用星形孢菌素预处理细胞可有效抑制其他促分泌素对ME分泌的长期刺激作用。此外，暴露于星形孢菌素24小时大大增加了前脑啡肽A（proENK）及其信使RNA（mRNA）的水平。星形孢菌素和PMA都以相似的程度增加了AP-1 DNA的结合活性。与星形孢菌素的结果相反，结构相似的化合物K252a（10 -8 M）没有显示出这些作用。此外，其他PKC抑制剂H7（10 -5 M）和鞘氨醇（3.6 x 10 -5 M），

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