• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleus >Lamin A/C is Expressed in Pluripotent Mouse Embryonic Stem Cells
【24h】

Lamin A/C is Expressed in Pluripotent Mouse Embryonic Stem Cells

机译：Lamin A / C在多能小鼠胚胎干细胞中表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of Lamin A/C contributes to these features. However, we show that mouse ESCs express low, yet readily detectable, amounts of Lamin A/C at both the RNA and protein levels. Full-length transcripts of both isoforms were readily detected by q-PCR and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of Lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESCs do express low levels of Lamin A/C, thus models linking pluripotency and nuclear dynamics with the absence of Lamin A/C need to be revisited.
机译：胚胎干细胞（ESC）的多能性与动态的开放染色质状态和不规则的核形状有关。据推测，没有Lamin A / C有助于这些功能。但是，我们显示，小鼠ESC在RNA和蛋白质水平上均表达低但易于检测的Lamin A / C量。通过q-PCR和深RNA测序很容易检测到两种同工型的全长转录本。此外，通过使用几种独立抗体的免疫印迹，在多个主要和已建立的ESC品系中验证了蛋白表达。免疫荧光标记显示Lamin A / C定位在所有检查的Oct4 / Nanog双阳性ESC系的核外围以及囊胚的内部细胞团中。我们的结果表明，ESC确实表达了低水平的Lamin A / C，因此需要重新研究在缺乏Lamin A / C的情况下将多能性和核动力学联系起来的模型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号