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Are genes switched on when they kiss?

机译：亲吻时基因会打开吗？

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摘要

Chromatin loops are pervasive and permit the tight compaction of DNA within the confined space of the nucleus. Looping enables distal genes and DNA elements to engage in chromosomal contact, to form multigene complexes. Advances in biochemical and imaging techniques reveal that loop-mediated contact is strongly correlated with transcription of interacting DNA. However, these approaches only provide a snapshot of events and therefore are unable to reveal the dynamics of multigene complex assembly. This highlights the necessity to develop single cell-based assays that provide single molecule resolution, and are able to functionally interrogate the role of chromosomal contact on gene regulation. To this end, high-resolution single cell imaging regimes, combined with genome editing approaches, are proving to be pivotal to advancing our understanding of loop-mediated dynamics.
机译：染色质环无处不在，并允许DNA在细胞核密闭空间内紧密压紧。环化使远端基因和DNA元件能够参与染色体接触，形成多基因复合体。生化和成像技术的进步表明，环介导的接触与相互作用DNA的转录密切相关。但是，这些方法仅提供事件的快照，因此无法揭示多基因复杂装配的动态。这凸显了开发基于单细胞的测定法的必要性，该测定法可提供单分子分辨率，并能够在功能上询问染色体接触在基因调控中的作用。为此，高分辨率单细胞成像方案与基因组编辑方法相结合，被证明对于增进我们对环介导的动力学的理解至关重要。

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