Eukaryotic genomes are organized into chromosomes. In order to maintain genomic stability during cell proliferation, a series of elaborate processes is employed to ensure that chromosomes are duplicated and segregated equally into daughter cells. Sister chromatid cohesion, a tight association of duplicated sister chromatids, allows their attachment to the opposite centrosomes. Sister chromatid cohesion depends on the cohesin complex, a proteinaceous ring that entraps the chromatids together. At the metaphase-to-anaphase transition, a protease called separase is activated and completely dissolves the cohesion by cleaving SCC1, a subunit of the cohesin complex. As one of the key executors of anaphase, separase is regulated temporally and spatially by often redundant mechanisms. A recent study revealed that chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur. This DNA dependence is the underlying biochemical mechanism that allows separase to selectively cleave only the chromosome- associated cohesin. We propose that the chromosomal DNA dependent cohesin cleavage by separase is a component of a regulatory pathway that cells utilize to protect the bulk of cohesin. This intact cohesin becomes immediately available in G1 to resume its other function - regulation of gene transcription by means of chromatin insulation.
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