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首页> 外文期刊>Laboratory investigation >Apoptosis and Apoptosis-Related Molecules in the Submandibular Gland of the Nonobese Diabetic Mouse Model for Sj|[ouml]|gren|[rsquo]|s Syndrome: Limited Role for Apoptosis in the Development of Sialoadenitis
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Apoptosis and Apoptosis-Related Molecules in the Submandibular Gland of the Nonobese Diabetic Mouse Model for Sj|[ouml]|gren|[rsquo]|s Syndrome: Limited Role for Apoptosis in the Development of Sialoadenitis

机译：Sj | [ouml] | gren | [rsquo] | s综合征的非肥胖糖尿病小鼠模型的颌下腺中的凋亡和与凋亡相关的分子：凋亡在唾液腺炎发展中的有限作用

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Sj?gren's syndrome is an autoimmune disease in which lymphocytic infiltrates develop in the exocrine glands. Pathogenetic aspects of the disease can be studied in the nonobese diabetic (NOD) mouse strain, a spontaneous model for Sj?gren's syndrome. Apoptosis may play a role in the initation phase and in the effector phase of autoimmune diseases. Here, we have examined the role of apoptosis in the development of sialoadenitis in the NOD mouse. Apoptotic cells and the expression of apoptosis-related molecules were studied in submandibular glands (SMG) of NOD and NOD-scid mice before and after the onset of sialoadenitis. Numbers of apoptotic cells were not increased as compared with control mice, at any age. By immunohistochemistry, we demonstrated increased expression of Fas, Fas ligand (FasL), and bcl-2 on SMG epithelial cells of NOD and NOD-scid mice, as early as 3 days of age. mRNA expression of Fas and FasL was also examined in SMG by RQ-PCR. Low-level expression of Fas and FasL mRNA was observed in all mouse strains, from 1 day of age onward. We conclude that increased protein expression of Fas and FasL on SMG epithelial cells of NOD and NOD-scid mice probably indicates a genetically programmed abnormality in these cells that may form a trigger for the development of sialoadenitis in NOD mice. Because increased numbers of apoptotic cells were not observed, a role for actual apoptosis in the initiation or effector phase of sialoadenitis in the NOD mouse is unlikely.
机译：干燥综合征是一种自身免疫性疾病，其中外分泌腺中出现淋巴细胞浸润。可以在非肥胖糖尿病（NOD）小鼠品系中研究该病的致病方面，该品系是Sjgren综合征的自发模型。细胞凋亡可能在自身免疫疾病的起始阶段和效应子阶段起作用。在这里，我们检查了凋亡在NOD小鼠唾液腺炎发展中的作用。研究了唾液腺炎发作前后NOD和NOD-scid小鼠下颌下腺（SMG）的凋亡细胞和凋亡相关分子的表达。在任何年龄，与对照小鼠相比，凋亡细胞的数量均没有增加。通过免疫组织化学，我们证明了早在3天大时，NOD和NOD-scid小鼠的SMG上皮细胞上Fas，Fas配体（FasL）和bcl-2的表达增加。还通过RQ-PCR检查了SMG中Fas和FasL的mRNA表达。从1日龄开始，在所有小鼠品系中均观察到Fas和FasL mRNA的低水平表达。我们得出结论，在NOD和NOD-scid小鼠的SMG上皮细胞上Fas和FasL的蛋白表达增加，可能表明这些细胞的遗传程序异常，可能会引发NOD小鼠唾液腺炎的发展。由于未观察到凋亡细胞数量的增加，因此在NOD小鼠的唾液腺炎的起始或效应期中实际凋亡的作用不太可能。

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