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首页> 外文期刊>FEBS Open Bio >Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.
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Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

机译：缺水引起的氧化应激影响青蒿中青蒿素的含量和脯氨酸代谢基因的表达。

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摘要

Water stress is one of the most critical abiotic stresses that restricts growth, development, and alters physiological and biochemical mechanisms of plant. The effects of long‐term water shortage‐induced oxidative stress on morphophysiological parameters, proline metabolic genes, and artemisinin content were studied in Artemisia annua L. under greenhouse conditions. Plant growth, biomass accumulation, relative water content, and chlorophyll content were reduced under drought. Leaf water potential ranged from ?0.3248 MPa to ?1.22 MPa in stress conditions. Increased levels of proline accumulation, protein concentration, and lipid peroxidation were detected in water‐stressed plants. Stage‐dependent increases in activity of antioxidants including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were observed. The expression of proline biosynthetic genes including pyrroline‐5‐carboxylase synthase1, 1‐pyrroline‐5‐carboxylase synthase2, and 1‐pyrroline‐5‐carboxylase reductase was induced, while the ornithine aminotransferase transcript showed a variable response and the expression of proline catabolic genes including proline dehydrogenase1, proline dehydrogenase1, and proline 5‐carboxylate dehydrogenase was reduced by water stress. Our results indicate that the glutamine pathway is predominant under drought stress in A. annua and a reduction of catabolic gene expression is adopted as a defense strategy in adverse conditions. Higher expression of biosynthetic genes and lower expression of catabolic genes at the preflowering stage confirmed the important role of proline in flower development. Artemisinin content decreased owing to water stress, but the slightly higher amounts were detected in leaves of severely stressed plants compared with moderately stressed plants. The artemisinin content of A. annua might be regulated by controlling irrigation regimes.
机译：水分胁迫是最关键的非生物胁迫之一，它会限制植物的生长，发育并改变植物的生理和生化机制。在温室条件下，研究了长期缺水引起的氧化应激对青蒿的形态生理参数，脯氨酸代谢基因和青蒿素含量的影响。干旱导致植物生长，生物量积累，相对含水量和叶绿素含量降低。在胁迫条件下，叶片水势在0.3248 MPa至1.22 MPa之间。在水分胁迫的植物中，脯氨酸的积累，蛋白质浓度和脂质过氧化水平增加。观察到抗氧化剂活性的阶段依赖性增加，包括超氧化物歧化酶，抗坏血酸过氧化物酶，谷胱甘肽还原酶，单脱氢抗坏血酸还原酶和脱氢抗坏血酸还原酶。诱导了脯氨酸生物合成基因的表达，包括吡咯啉-5-羧化酶合酶1、1-吡咯啉-5-羧化酶合酶2和1-吡咯啉-5-羧化酶还原酶，而鸟氨酸氨基转移酶转录物表现出可变的响应，脯氨酸分解代谢的表达水分胁迫降低了脯氨酸脱氢酶1，脯氨酸脱氢酶1，脯氨酸5-羧酸酯脱氢酶等基因的表达。我们的结果表明，谷氨酸途径在干旱条件下在青蒿中是主要的，并且分解代谢基因表达的减少被用作不利条件下的防御策略。在开花前，生物合成基因的较高表达和分解代谢基因的较低表达证实了脯氨酸在花发育中的重要作用。青蒿素含量由于水分胁迫而降低，但与中度胁迫植物相比，重度胁迫植物的叶片中检出的青蒿素含量略高。青蒿的青蒿素含量可以通过控制灌溉制度来调节。

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