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首页> 外文期刊>FEBS Open Bio >The widespread occurrence of tRNA‐derived fragments in Saccharomyces cerevisiae
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The widespread occurrence of tRNA‐derived fragments in Saccharomyces cerevisiae

机译：酿酒酵母中tRNA来源片段的广泛存在

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摘要

Short RNAs derived from the cleavage of tRNA molecules are observed in most organisms. Their occurrence seems to be induced by stress conditions, but still little is known about their biogenesis and functions. We find that the recovery of tRNA fragments depends on the RNA isolation method. Using an optimized RNA extraction protocol and northern blot hybridization technique, we show that the tRNA‐derived fragments in yeast are widespread in 12 different growth conditions. We did not observe significant stress‐dependent changes in the amounts of tRNA fragments pool. Instead, we show the differential processing of almost all individual tRNAs. We also provide evidence that 3′‐part‐derived tRNA fragments are as abundant as the 5′‐ one in Saccharomyces cerevisiae. The resulting set of S. cerevisiae tRNA fragments provides a robust basis for further experimental studies on biological functions of tRFs.
机译：在大多数生物中都观察到源自tRNA分子裂解的短RNA。它们的发生似乎是由压力条件引起的，但对其生物发生和功能了解甚少。我们发现，tRNA片段的回收取决于RNA分离方法。使用优化的RNA提取方案和Northern blot杂交技术，我们证明了酵母中tRNA衍生的片段在12种不同的生长条件下广泛分布。我们在tRNA片段库的数量上没有观察到明显的压力依赖性变化。相反，我们显示了几乎所有单个tRNA的差异处理。我们还提供了证据，证明酿酒酵母中3'来源的tRNA片段与5'-1一样丰富。所得啤酒酵母tRNA片段组为进一步研究tRF的生物学功能提供了坚实的基础。

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