Two epitopes responsible for the catalytic activity of heme oxygenase‐1 identified by phage display

Xuran Wei; Qingjun Liu; Yaping Gao; Jun Yang; Bo Wang; Guang Yang; Shihui Zhang; Hong Zhou

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Two epitopes responsible for the catalytic activity of heme oxygenase‐1 identified by phage display

机译：通过噬菌体展示鉴定出负责血红素加氧酶-1催化活性的两个表位

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Heme oxygenase‐1 (HO‐1) catalyzes the oxidative degradation of heme. The catalytic mechanism of the HO‐1 reaction has been determined gradually by studies of its crystal structure and HO‐1 mutants. However, the neutralizing epitopes responsible for HO‐1 activity remain elusive. Screening of a phage display library revealed four epitopes that could interact with the polyclonal antibody prepared by immunizing rabbits with the purified HO‐1 protein. Two of these four epitopes are responsible for HO‐1 catalytic activity because their antibodies were able to neutralize HO‐1 activity. The results of the present study shed further light on the molecular character of HO‐1.

机译：血红素加氧酶-1（HO-1）催化血红素的氧化降解。 HO-1反应的催化机理已通过研究其晶体结构和HO-1突变体逐渐确定。但是，负责HO-1活性的中和表位仍然难以捉摸。噬菌体展示文库的筛选揭示了四个表位，这些表位可能与通过用纯化的HO-1蛋白免疫兔而制备的多克隆抗体相互作用。这四个表位中的两个负责HO-1催化活性，因为它们的抗体能够中和HO-1活性。本研究的结果进一步揭示了HO-1的分子特征。

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《FEBS Open Bio》 |2017年第5期|共8页
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Xuran Wei; Qingjun Liu; Yaping Gao; Jun Yang; Bo Wang; Guang Yang; Shihui Zhang; Hong Zhou;
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中图分类生物化学;
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机译：通过噬菌体上随机肽库鉴定链激酶抗原表位的序列。
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机译：犬内脏利什曼病的减法噬菌体展示选择确定了模仿婴儿利什曼原虫抗原的新型表位，并具有潜在的血清学诊断应用
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机译：随机诱变和噬菌体展示技术作为鉴定桦树花粉过敏原Bet v 1的ige表位的工具
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机译：EPR表征血红素氧酶反应中间体及其对催化机制的影响
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机译：确定负责人血红素加氧酶基因的镉介导的顺式作用元件
6. Two epitopes responsible for the catalytic activity of heme oxygenase‐1 identified by phage display [O] . Xuran Wei, Qingjun Liu, Yaping Gao, -1

机译：通过噬菌体展示鉴定出两个负责血红素加氧酶-1催化活性的表位
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机译：DNA免疫与scFv噬菌体展示相结合可识别拮抗的GCGR特异性抗体，并揭示小细胞外环上的新表位
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机译：大鼠肝脏δ-氨基乙酰丙酸合成酶和血红素加氧酶活性的调节：血红素控制和假体铁调节的证据

Two epitopes responsible for the catalytic activity of heme oxygenase‐1 identified by phage display

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