...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEBS Open Bio >Down‐regulation of miR‐26b induces cisplatin resistance in nasopharyngeal carcinoma by repressing JAG1
【24h】

Down‐regulation of miR‐26b induces cisplatin resistance in nasopharyngeal carcinoma by repressing JAG1

机译：下调miR-26b通过抑制JAG1诱导鼻咽癌顺铂耐药

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Therapy against nasopharyngeal carcinoma (NPC) is hurdled by chemoresistance. Recent studies found that microRNA (miRNA) are important regulators of cancer resistance. In this study, we aimed to explore the role and mechanism of miR‐26b in regulating NPC cisplatin (CDDP) resistance. Real‐time PCR was used to evaluate miR‐26b levels in CDDP‐resistant and CDDP‐sensitive NPC cells, as well as human NPC tissues. MiR‐26b was ectopically overexpressed in CDDP‐resistant cells, followed by monitoring changes in cell viability and apoptosis. Interaction between JAG1 and miR‐26b was characterized by dual‐luciferase reporter assay. Furthermore, we investigated whether ectopic JAG1 expression reversed CDDP sensitivity induced by miR‐26b overexpression. The effect of FOXD3 down‐regulation on miR‐26b was also evaluated. Our results indicate that miR‐26b was lower in the CDDP‐resistant NPC cells, human NPC tissue, particularly in secondary metastases. Ectopic expression of miR‐26b sensitized NPC cells to CDDP. JAG1 is a target of miR‐26b, and its expression is inversely correlated with miR‐26b. Overexpression of JAG1 reversed the CDDP sensitivity induced by miR‐26b overexpression. FOXD3 expression was also down‐regulated in CDDP‐resistant NPC. FOXD3 promoted miR‐26b expression and down‐regulation of FOXD3 suppressed miR‐26b expression. Down‐regulation of miR‐26b is closely correlated with the CDDP resistance in NPC.
机译：化学抗药性阻碍了对鼻咽癌（NPC）的治疗。最近的研究发现，microRNA（miRNA）是抗癌性的重要调节剂。在这项研究中，我们旨在探讨miR‐26b在调节NPC顺铂（CDDP）耐药性中的作用和机制。实时荧光定量PCR用于评估CDDP耐药和CDDP敏感的NPC细胞以及人NPC组织中的miR-26b水平。 MiR‐26b在CDDP耐药细胞中异位表达，然后监测细胞活力和凋亡的变化。 JAG1和miR‐26b之间的相互作用通过双荧光素酶报告基因分析进行了表征。此外，我们研究了异位JAG1表达是否逆转了miR-26b过表达诱导的CDDP敏感性。还评估了FOXD3下调对miR-26b的影响。我们的结果表明，在耐CDDP的NPC细胞，人NPC组织中，miR‐26b较低，尤其是在继发转移中。 miR‐26b敏化的NPC细胞对CDDP的异位表达。 JAG1是miR‐26b的靶标，其表达与miR‐26b成反比。 JAG1的过表达逆转了miR-26b过表达诱导的CDDP敏感性。 FOXD3表达在耐CDDP的NPC中也下调。 FOXD3促进miR-26b表达，而FOXD3的下调抑制了miR-26b表达。 miR-26b的下调与NPC中的CDDP抗性密切相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号