Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis

James Jung; Ghader Bashiri; Jodie M. Johnston; Edward N. Baker

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Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis

机译：质谱法测定结核分枝杆菌中载体蛋白的磷酸泛肽基化特异性

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Phosphopantetheinyl transferases (PPTases) are key elements in the modular syntheses performed by multienzyme systems such as polyketide synthases. PPTases transfer phosphopantetheine derivatives from Coenzyme A to carrier proteins (CPs), thus orchestrating substrate supply. We describe an efficient mass spectrometry‐based protocol for determining CP specificity for a particular PPTase in organisms possessing several candidate PPTases. We show that the CPs MbtL and PpsC, both involved in synthesis of essential metabolites in Mycobacterium tuberculosis, are exclusively activated by the type 2 PPTase PptT and not the type 1 AcpS. The assay also enables conclusive identification of the reactive serine on each CP.

机译：磷酸泛肽基转移酶（PPTase）是由多酶系统（如聚酮化合物合酶）进行的模块合成中的关键要素。 PPTase将磷酸泛素衍生物从辅酶A转移到载体蛋白（CP），从而协调底物的供应。我们描述了一种有效的基于质谱的方案，用于确定具有几种候选PPTase的生物体中特定PPTase的CP特异性。我们显示CPs MbtL和PpsC，都参与结核分枝杆菌中基本代谢产物的合成，仅由2型PPTase PptT而非1型AcpS激活。该测定还能够确定性鉴定每个CP上的反应性丝氨酸。

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《FEBS Open Bio》 |2016年第12期|共7页
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James Jung; Ghader Bashiri; Jodie M. Johnston; Edward N. Baker;
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中图分类生物化学;
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1. Phosphopantetheinylation and Specificity of Acyl Carrier Proteins in the Mupirocin Biosynthetic Cluster [J] . Shields JA, Rahman AS, Arthur CJ, Chembiochem: A European journal of chemical biology . 2010,第2期

机译：Mupirocin生物合成簇中磷酸泛素化和酰基载体蛋白的特异性。
2. In silico Analysis and Experimental Validation of Mycobacterium tuberculosis-Specific Proteins and Peptides of Mycobacterium tuberculosis for Immunological Diagnosis and Vaccine Development [J] . Mustafa A.S. Medical principles and practice: international journal of the Kuwait University, Health Science Centre . 2013,第Supplaa1期

机译：结核分枝杆菌特异性蛋白和多肽的免疫分析和疫苗开发的计算机分析和实验验证
3. Decrypting the oscillating nature of the 4′‐phosphopantetheine arm in acyl carrier protein AcpM of Mycobacterium?tuberculosis Mycobacterium?tuberculosis [J] . Biswas Rupam, Singh Bina Kumari, Dutta Debajyoti, FEBS letters. . 2019,第6期

机译：解压缩4'-磷酸乙醚臂的振荡性质在分枝杆菌的酰基载体蛋白ACPM中的α结核分枝杆菌？结核病
4. Machine learning approaches for customized docking scores: Modeling of inhibition of Mycobacterium tuberculosis enoyl acyl carrier protein reductase [C] . Fogel Gary B., Tran Jonathan, Johnson Stephen, 2010 IEEE Symposium on Computational Intelligence in Bioinformatics and Computational Biology . 2010

机译：用于定制对接分数的机器学习方法：结核分枝杆菌烯酰基酰基载体蛋白还原酶抑制模型
5. Comparative analyses of transport proteins encoded within the genomes of Mycobacterium tuberculosis and Mycobacterium leprae. [D] . Youm, Ji-Won. 2008

机译：结核分枝杆菌和麻风分枝杆菌基因组内编码的转运蛋白的比较分析。
6. Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis [O] . James Jung, Ghader Bashiri, Jodie M. Johnston, -1

机译：质谱法测定结核分枝杆菌中载体蛋白的磷酸泛肽基化特异性
7. Pesquisa de IgA contra o antígeno recombinante HspX de Mycobacterium tuberculosis no diagnóstico de tuberculose pleural Determination of levels of specific IgA to the HspX recombinant antigen of Mycobacterium tuberculosis for the diagnosis of pleural tuberculosis [O] . Loanda Carvalho Sant' Ana Limongi, Liliane Olival, Marcus Barreto Conde, 2011

机译：重组结核分枝杆菌HspX抗原Iga研究对胸膜结核的诊断价值

Mass spectral determination of phosphopantetheinylation specificity for carrier proteins in Mycobacterium tuberculosis

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