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首页> 外文期刊>FEBS Open Bio >Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population
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Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

机译：匈牙利人群口腔鳞状细胞癌的唾液蛋白质组学分析

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Oral squamous cell carcinoma (OSCC) is the seventh most common malignancy and the ninth most frequent cause of cancer death in Europe. Within Europe, Hungary has one of the highest rates of OSCC incidence and mortality. Thus, there is an urgent need to improve early detection. Saliva, as a readily available body fluid, became an increasingly important substance for the detection of biomarkers for many diseases. Different research groups have identified salivary biomarkers specific for OSCC for different countries. In this study, saliva samples of Hungarian patients with OSCC were studied to discover disease‐specific and perhaps region‐specific biomarkers. LC‐mass spectrometry (MS)/MS analysis on a linear ion trap‐Orbitrap mass spectrometer was used for qualitative and quantitative salivary protein profiling. More than 500 proteins were identified from saliva by shotgun proteomics. The up‐ and downregulated proteins in the saliva of patients with OSCC highlighted the importance of protein–protein interaction networks involving the immune system and proteolysis in disease development. Two potential biomarkers from our shotgun analysis and a third candidate reported earlier by a Taiwanese group were further examined by ELISA on a larger reference set of samples. Resistin, a biomarker reported in Taiwan but not validated in our study, highlights the necessity of application of standardized analysis methods in different ethnic or geographical populations to identify biomarkers with sufficient specificity and sensitivity.
机译：口腔鳞状细胞癌（OSCC）是欧洲第七大最常见的恶性肿瘤，也是第九大最常见的癌症死亡原因。在欧洲，匈牙利是OSCC发病率和死亡率最高的国家之一。因此，迫切需要改进早期检测。唾液作为一种随时可用的体液，已成为检测多种疾病生物标志物的越来越重要的物质。不同的研究小组已经确定了针对不同国家OSCC的唾液生物标志物。在这项研究中，对匈牙利患有OSCC的患者的唾液样本进行了研究，以发现疾病特异性生物标志物，也许还发现了区域特异性生物标志物。线性离子阱Orbitrap质谱仪上的LC-质谱（MS）/ MS分析用于定性和定量唾液蛋白分析。通过shot弹枪蛋白质组学从唾液中鉴定出500多种蛋白质。 OSCC患者唾液中蛋白质的上调和下调突出了涉及疾病发展过程中涉及免疫系统和蛋白水解作用的蛋白质-蛋白质相互作用网络的重要性。通过ELISA在更大的参考样本集中进一步检查了我们shot弹枪分析中的两种潜在生物标志物以及台湾小组先前报告的第三种候选物。 Resistin是台湾报道的一种生物标志物，但未在我们的研究中得到验证。它强调了在不同种族或地理区域中应用标准化分析方法来鉴定具有足够特异性和敏感性的生物标志物的必要性。

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