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首页> 外文期刊>FEBS Open Bio >Disperse Orange 3 as a resonance Raman probe for measuring membrane order
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Disperse Orange 3 as a resonance Raman probe for measuring membrane order

机译：分散Orange 3作为共振拉曼探针以测量膜的阶数

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Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO"2 symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4cm^-^1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.
机译：在脂质膜存在下检查了偶氮苯衍生物的共振拉曼光谱，以找到可以区分不同膜相的探针。 4-（4-硝基苯基偶氮）苯胺的NO“ 2对称拉伸带，也称为分散橙3（DO3），在磷脂酰胆碱膜从液晶到凝胶的相变中下移约4cm ^-^ 1当DO3在液相阶段与含胆固醇的膜结合时，也会发生类似的降档，我们的结果表明DO3的拉曼光谱是测量膜中脂质分子顺序的独特工具。

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