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首页> 外文期刊>FEBS Open Bio >Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis
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Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis

机译：定量PCR分析揭示了对各种小鼠组织中1天或2天禁食的差异适应性反应

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Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (Atrogin-1 and MuRF1), autophagy-lysosome (LC3b, p62 and Lamp2), amino acid response (Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (HO-1 and Gsta1), and amino acid transport (Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
机译：饮食或热量限制可带来各种临床益处。小鼠的短期禁食是一种常见的实验程序，可能涉及系统性代谢重塑，这可能会严重影响实验输出。这项研究通过定量PCR使用15种标记物引物组对遍在蛋白蛋白酶体（Atrogin-1和MuRF1），自噬溶酶体（LC3b，p62和Lamp2）的激活进行定量PCR，评估了13个小鼠组织禁食1天或2天后的适应性细胞反应。 ），氨基酸响应（Asns，Trib3，Herpud1，xCT和Chop），Nrf2介导的抗氧化剂（HO-1和Gsta1）和氨基酸转运（Slc38a2，Slc7a5和Slc7a1）系统。在七个高度（胸腺，肝脏，脾脏和小肠）或轻度（胃，肾脏和结肠）萎缩的组织以及六个非萎缩的组织（大脑，眼睛，肺，心脏，骨骼肌）中获得差异激活谱和睾丸）建议进行组织特异性主动代谢重建。

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