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Mature adipocytes observed to undergo reproliferation and polyploidy

机译:观察到成熟脂肪细胞发生增殖和多倍性

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Lipid‐filled mature adipocytes are important for the study of lipid metabolism and in the development of obesity, but whether they are capable of reproliferation is still controversial. Here, we monitored lipid droplet dynamics and adipocyte reproliferation in live, differentiated 3T3‐L1 cells using a phase‐contrast microscope in real time. Phase‐contrast microscopy achieves a similar visual effect in situ to that obtained using traditional dyes such as Oil Red O and BODIPY in vitro. Using this method, we captured the process that lipid droplets use for dynamic fusion in living cells. Unexpectedly, we acquired images of the moment that differentiated 3T3‐L1 cells containing lipid droplets entered mitosis. In addition, we observed some binucleated mature adipocytes. This information provides a better understanding of the adipocyte differentiation process.
机译:充满脂质的成熟脂肪细胞对于脂质代谢研究和肥胖症的发展非常重要,但是它们是否能够增殖仍然存在争议。在这里,我们使用相差显微镜实时监测了分化的活3T3-L1细胞中脂滴的动态和脂肪细胞的增殖。相衬显微镜在体外可达到与使用传统染料(如油红O和BODIPY)所获得的视觉效果相似的视觉效果。使用这种方法,我们捕获了脂质滴在活细胞中动态融合的过程。出乎意料的是,我们获得了包含脂质小滴的3T3-L1细胞分化进入有丝分裂这一瞬间的图像。此外,我们观察到一些双核成熟脂肪细胞。该信息提供了对脂肪细胞分化过程的更好理解。

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