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首页> 外文期刊>Reproductive Biology and Endocrinology >Effect of electro-acupuncture on ovarian expression of α (1)- and β (2)-adrenoceptors, and p75 neurotrophin receptors in rats with steroid-induced polycystic ovaries
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Effect of electro-acupuncture on ovarian expression of α (1)- and β (2)-adrenoceptors, and p75 neurotrophin receptors in rats with steroid-induced polycystic ovaries

机译：电针对激素性多囊卵巢大鼠卵巢α（1）和β（2）肾上腺素能受体及p75神经营养因子受体表达的影响。

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Background Estradiol valerate (EV)-induced polycystic ovaries (PCO) in rats is associated with an increase in ovarian sympathetic outflow. Low-frequency (2 Hz) electro-acupuncture (EA) has been shown to modulate sympathetic markers as well as ovarian blood flow as a reflex response via the ovarian sympathetic nerves, in rats with EV-induced PCO. Methods In the present study, we further tested the hypothesis that repeated 2 Hz EA treatments modulate ovarian sympathetic outflow in rats with PCO, induced by a single i.m. injection of EV, by investigating the mRNA expression, the amount and distribution of proteins of α1a-, α1b-, α1d-, and β2-adrenoceptors (ARs), as well as the low-affinity neurotrophin receptor (p75NTR). Results It was found that EV injection results in significantly higher mRNA expression of ovarian α1b- and α1d-AR in PCO rats compared to control rats. The p75NTR and β2-ARs mRNA expression were unchanged in the PCO ovary. Low-frequency EA resulted in a significantly lower expression of β2-ARs mRNA expression in PCO rats. The p75NTR mRNA was unaffected in both PCO and control rats. PCO ovaries displayed significantly higher amount of protein of α1a-, α1b- and α1d-ARs, and of p75NTR, compared to control rats, that were all counteracted by repeated low-frequency EA treatments, except for α1b-AR. Conclusion The present study shows that EA normalizes most of the EV-induced changes in ovarian ARs. Furthermore, EA was able to prevent the EV-induced up regulation of p75NTR, probably by normalizing the sympathetic ovarian response to NGF action. Our data indicate a possible role of EA in the regulation of ovarian responsiveness to sympathetic inputs and depict a possible complementary therapeutic approach to overcoming sympathetic-related anovulation in women with PCOS.
机译：背景戊酸雌二醇（EV）诱导的大鼠多囊卵巢（PCO）与卵巢交感神经外流增加有关。低频（2 Hz）电针（EA）已被证明可在通过EV诱发的PCO的大鼠中通过卵巢交感神经调节交感神经标记以及卵巢血流的反射反应。方法在本研究中，我们进一步验证了以下假设：重复2 Hz EA治疗可调节由单次i.m.诱发的PCO大鼠卵巢交感神经外流。通过研究α1a-，α1b-，α1d-和β2-肾上腺素能受体（ARs）以及低亲和力神经营养蛋白受体（p75NTR）的mRNA表达，蛋白的量和分布来注射EV。结果发现，与对照组相比，EV注射导致PCO大鼠卵巢α1b-和α1d-AR的mRNA表达明显升高。在PCO卵巢中，p75NTR和β2-ARs的mRNA表达没有变化。低频EA导致PCO大鼠中β2-ARsmRNA表达明显降低。在PCO和对照大鼠中，p75NTR mRNA均不受影响。与对照大鼠相比，PCO卵巢显示出的α1a-，α1b-和α1d-ARs和p75NTR蛋白质的含量显着更高，除α1b-AR外，所有这些都被重复的低频EA治疗所抵消。结论本研究表明，EA能使大多数由EV引起的卵巢ARs改变正常化。此外，EA可能通过使对NGF作用的交感卵巢反应正常化，从而阻止了EV诱导的p75NTR上调。我们的数据表明，EA在调节卵巢对交感神经反应的反应中可能发挥作用，并描绘了可能的补充治疗方法，以克服PCOS妇女的交感相关无排卵。

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