• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Research Journal of Medical Sciences >Is Rotavirus Infection Still Responsible for Acute Gastroenteritis and Severe Diarrhea among Children in Holy Makkah?
【24h】

Is Rotavirus Infection Still Responsible for Acute Gastroenteritis and Severe Diarrhea among Children in Holy Makkah?

机译：在圣麦加儿童中，轮状病毒感染是否仍对儿童急性胃肠炎和严重腹泻负责？

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acute gastroenteritis and severe diarrhea is a leading cause of preventable death in infants and young children worldwide. It ranks second to neonatal deaths as the major cause of childhood mortality. In this regard, rotavirus infection is the most important microbial causative agent, particularly in developing countries. Coherently, this study was designed to assess the prevalence of rotavirus infection among young children with acute gastroenteritis and severe diarrhea in Holy Makkah, Saudi Arabia and evaluate the specificity of Latex Agglutination Test (LAT) in detection of rotavirus infection in comparison with Reverse-Transcription Polymerase Chain Reaction (RT-PCR). During the period from March to September 2011, stool samples were collected from 100 children (of both sex and under 5 years of age) with acute gastroenteritis and severe diarrhea and admitted to the pediatric wards at the Garwal Hospital in Holly Makkah. For detection of rotavirus infection, the samples were first examined by LAT and then by RT-PCR using specific primer sequences for human rotavirus VP4 and VP7 genes. Sixteen samples of these 100 samples (16%) were positive for rotavirus by LAT. However, the genetic materials of rotavirus (VP4 and VP7 genes) were only detected in 4 samples of these 100 samples (4%) by RT-PCR amplification. The samples that tested negative with LAT were also negative with RT-PCR. Overall, this study indicated that rotavirus infection still responsible for acute gastroenteritis and severe diarrhea among children in Holy Makkah with a prevalence of 4%. In turn, this may help in assessing the success of the rotavirus vaccine in the future. In addition, this study reflects the low specificity of LAT than RT-PCR for detection of rotavirus infection.
机译：急性胃肠炎和严重腹泻是世界范围内可预防的死亡的主要原因。它是导致儿童死亡的主要原因，仅次于新生儿死亡。在这方面，轮状病毒感染是最重要的微生物病原体，在发展中国家尤其如此。一致地，本研究旨在评估沙特阿拉伯圣麦加患有急性胃肠炎和严重腹泻的幼儿中轮状病毒感染的患病率，并评估乳胶凝集试验（LAT）在检测轮状病毒感染中的特异性与逆转录比较聚合酶链反应（RT-PCR）。在2011年3月至2011年9月期间，从100名患有急性胃肠炎和严重腹泻的儿童（性别和5岁以下）中收集了粪便样本，并送往Holly Makkah的Garwal医院的儿科病房。为了检测轮状病毒感染，首先使用人轮状病毒VP4和VP7基因的特异性引物序列通过LAT，然后通过RT-PCR检查样品。在这100个样品中，有16个样品（占16％）的LAT轮状病毒呈阳性。然而，通过RT-PCR扩增仅在这100个样品中的4个样品中检测到轮状病毒的遗传物质（VP4和VP7基因）（4％）。用LAT呈阴性的样品在RT-PCR中也呈阴性。总体而言，这项研究表明，圣麦加地区儿童中，轮状病毒感染仍是导致急性肠胃炎和严重腹泻的原因，患病率为4％。反过来，这可能有助于将来评估轮状病毒疫苗的成功。此外，这项研究反映了LAT的特异性低于轮状病毒感染的RT-PCR。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号