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首页> 外文期刊>Research Journal of Medical Sciences >Application of Haradi Mori?s Culture Method for Differentiating Hookworm Species in Two Major Cities of Southern Nigeria
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Application of Haradi Mori?s Culture Method for Differentiating Hookworm Species in Two Major Cities of Southern Nigeria

机译：Haradi Mori的培养方法在尼日利亚南部两个主要城市的钩虫种类鉴别中的应用

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The study was undertaken to differentiate hookworm species in Owerri and Port Harcourt during late rainy and early dry seasons, by the application of Harada-mori culture methods. Six hundred faecal. Samples (300 in each of the cities) were screened for positivity of the hookworm ova. Prevalence of hookworm infection was statistically higher in Owerri (23.3%) than Port Harcourt (13.3%, p< 0.005) and this was related to seasons of study. Sections of the cities with poor drainage and lower standard of hygiene (Diobu, Port Harcourt and Amakohia, Owerri) had higher prevalence rates (8%, 11.6%respectively) than those zones (Main towns) with improved standard of sanitation and adequate drainage facilities (prevalence rates: 1.3 and 3.3%, respectively). Thirty five of the fourty positive samples (87.5%) in Port Harcourt revealed the filariform larvae (second larval stage) of Necator americanus 25.5% were Ancylostoma duodenale: Similarly, 63 sixty three of the seventy positive samples (90%) obtained from Owerri were Necator americanus as against 10% which were A. duodenale. Both species were harvested from culture of fresh hookworm positive stool samples in Harada-mori apparatus for a period of ten days at room temperature (25-28?C). Predominance of N. americanus confirms with previous studies and appears to re-enforce the efficiency of Harada-mori culture method. The method was simple, cost effective and required minimum skills. Relative endemic status of the study areas were highlighted.
机译：这项研究是通过应用原田森栽培技术来区分雨季后期和干旱初期的Owerri和Harcourt港的钩虫种类。六百粪便。筛选样本（每个城市300个）的钩虫卵阳性。 Owerri（23.3％）的钩虫感染发生率在统计学上高于Harcourt港（13.3％，p <0.005），这与研究季节有关。排水较差且卫生水平较低的城市部分（Diobu，Harcourt港和Owerri的Amakohia）的患病率（分别为8％，11.6％）高于那些卫生条件得到改善且排水设施充足的地区（主要城镇） （患病率分别为1.3％和3.3％）。哈科特港的四十个阳性样本中有三十五个（87.5％）揭示了美国Necator的丝状幼虫（第二个幼虫阶段）为25.5％，是十二指肠成虫：同样，从Owerri获得的七十个阳性样本中有六十三个（90％）为美洲轮齿草占10％，而美国十二指肠菌占10％。在室温（25-28℃）下，在原田森装置中从新鲜钩虫阳性粪便样品中培养十天，收获了这两个物种。美洲猪笼草的优势在先前的研究中得到证实，并且似乎增强了原田森文化方法的效率。该方法简单，具有成本效益并且需要最低技能。研究区域的相对流行状况被强调。

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