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首页> 外文期刊>Research Journal of Medical Sciences >Total Reendothelialization of Vascular Grafts by Circulating Recipient Endothelial Stem Cells in a Transgenic Green Rat
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Total Reendothelialization of Vascular Grafts by Circulating Recipient Endothelial Stem Cells in a Transgenic Green Rat

机译：循环移植受者内皮干细胞在转基因绿色大鼠中的血管移植物的总内皮重。

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This study was designed to unravel if arterial grafts treated with phosphate buffered saline or 10Gy irradiation to induce endothelial cell loss, still contain enough biological information to drive proper endothelial regeneration. To demonstrate that damage to donor arteries retains the biological information needed to drive proper differentiation of circulating endothelial precursor cells, arteries were either irradiated (n=10) (10Gy) or stored for 30 min in PBS (n=10) at 20 C. After treatment the arteries were grafted end to end in the aorta descendens of GFP transgenic rats. Three weeks after implantation 5 grafts were recovered and the remaining 5 after 6 weeks and were analyzed immuno histochemically using antibodies to endothelial cell lineage markers (CD31 and von Willebrand factor), Griffiona simplicifolia lectin and Green Fluorescent Protein (GFP). Arteries processed immediately after surgery served as control. Grafted arteries had an intact endothelial layer. Three weeks after graft implantation the arteries were totally denuded for both treatment protocols, while cells attach to the fibroelastic layer. Six weeks after grafting the grafts showed neointima formation and were totally reendothelialized with recipient cells. The fibroelastic layer and adventitia also contained green recipient cells. These results provide compelling evidence that mild treated arteries loose their endothelial lining but still contain the biological information to drive endothelial differentiation of recipient circulating endothelial precursor cells resulting in a intact endothelial layer six weeks after surgery. This in contrast to harsh treated grafts.
机译：这项研究旨在揭示是否用磷酸盐缓冲盐水或10Gy辐射处理的动脉移植物诱导内皮细胞损失，但仍包含足够的生物学信息来驱动正常的内皮细胞再生。为了证明对供体动脉的损伤保留了驱动正常循环的内皮前体细胞分化所需的生物学信息，对动脉进行了辐照（n = 10）（10Gy）或在20°C的PBS中存储30分钟（n = 10）。治疗后，将动脉首尾相接地移植到GFP转基因大鼠的主动脉后肢中。植入后三周，恢复了5个移植物，其余5个在6周后恢复，并使用抗内皮细胞谱系标志物（CD31和von Willebrand因子），Griffiona simplicifolia lectin和绿色荧光蛋白（GFP）的抗体进行了免疫组织化学分析。手术后立即处理的动脉作为对照。嫁接的动脉具有完整的内皮层。移植物植入三周后，两种治疗方案都完全剥夺了动脉，而细胞则附着在纤维弹性层上。移植后六周，移植物显示出新内膜形成，并与受体细胞完全重新内皮化。纤维弹性层和外膜也含有绿色受体细胞。这些结果提供了令人信服的证据，表明轻度治疗的动脉会松动其内皮内膜，但仍包含生物学信息以驱动受体循环的内皮前体细胞的内皮分化，从而在手术后六周形成完整的内皮层。这与经过严格处理的移植物相反。

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