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首页> 外文期刊>Research Journal of Immunology >Development of Gamma Irradiation Vaccine against Mannheimia haemolytica : A Preliminary Study
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Development of Gamma Irradiation Vaccine against Mannheimia haemolytica : A Preliminary Study

机译：溶血曼氏疟原虫γ辐射疫苗的初步研究。

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The study aimed to use the several advantages of nuclear techniques for developing irradiation vaccine against Mannheimia haemolytica using different gamma radiation doses for vaccines preparation and different inoculation doses of irradiation vaccine. The M. haemolytica was exposed to different doses of gamma radiation. The dose rate was considered the optimum irradiating dose that was Lethal to M. haemolytica cells and selected for optimal gamma irradiation vaccine. Experimental animals were divided into four groups. The experimental groups injected twice with three weeks interval for tested vaccines. The first group (G1) inoculated with 4×109 bacterial cells/dose from optimum irradiation vaccine. The second group (G2) inoculated with 2×109 bacterial cells/dose from optimum irradiation vaccine. The third group (G3) inoculated with 4×109 bacterial cells/dose from high irradiation vaccine. The fourth group (C) injected (S/C) with 2 mL sterile PBS and was kept as a control group. Vaccination challenge with wild M. haemolytica life organism (0.5 mL of 3.6×1010 mL-1) was two doses for all experimental animals. ELISA was used to evaluate the efficiency of vaccines. The antibodies production are evaluated using Optical Density (OD) value as an indication of the efficiency of vaccine against M. haemolytica . The results revealed that after the second vaccination dose, the OD value of G2 showed a significant difference compared to G1 and G3 groups and it was non-significant between G1 and G3 groups. Comparative analysis of control and the different doses of gamma irradiation vaccines showed that after the second vaccination dose, the mean of OD value of the G2 was a significant different while it was non-significant in the G1and G3 compared to the control group. After vaccination challenge, the mean of OD value of G2 was with high significant different compared to all of vaccinated and control groups.
机译：该研究旨在利用核技术的几个优势来开发针对溶血曼海姆氏菌的辐射疫苗，使用不同的伽马辐射剂量制备疫苗，并使用不同接种剂量的辐射疫苗。溶血支原体暴露于不同剂量的伽马射线。剂量率被认为是对溶血支原体细胞致死的最佳照射剂量，并被选择用于最佳伽马射线照射疫苗。实验动物分为四组。实验组以三周的间隔注射两次以测试疫苗。第一组（G 1 ）接种了最佳辐射疫苗接种的4×10 9 细菌细胞/剂量。第二组（G 2 ）接种了最佳辐射疫苗接种的2×10 9 细菌细胞/剂量。第三组（G 3 ）接种高辐射疫苗接种的4×10 9 细菌细胞/剂量。第四组（C）注射（S / C）2 mL无菌PBS，并作为对照组。野生溶血支原体生命生物（0.5 mL的3.6×10 10 mL -1 ）进行的疫苗接种对所有实验动物都是两次剂量。 ELISA用于评估疫苗的效率。使用光密度（OD）值作为抗溶血支原体疫苗效力的指标来评估抗体的产生。结果表明，第二次接种疫苗后，G 2 的OD值与G 1 和G 3 组相比有显着差异，并且G 1 和G 3 组之间的差异不显着。对照和不同剂量γ射线疫苗的对比分析表明，第二次接种疫苗后，G 2 的OD平均值具有显着差异，而G 2 则无统计学意义。与对照组相比，SUP> 1 和G 3 。接种疫苗后，与所有疫苗接种组和对照组相比，G 2 的OD平均值具有显着差异。

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