• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Resource-Efficient Technologies >Effect of functionalization of polymeric nanoparticles incorporated with whole attenuated rabies virus antigen on sustained release and efficacy
【24h】

Effect of functionalization of polymeric nanoparticles incorporated with whole attenuated rabies virus antigen on sustained release and efficacy

机译：掺有完整减毒狂犬病毒抗原的聚合物纳米颗粒的功能化对缓释和功效的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nanovaccines introduced a new dimension to prevent or cure diseases in an efficient and sustained manner. Various polymers have been used for the drug delivery to increase the therapeutic value with minimal side effects. Thus the present study incorporates both nanotechnology and polymers for the drug delivery. Poly( d , l -lactic-co-glycolic acid)-b-poly(ethylene glycol) was incorporated with the rabies whole attenuated viral antigen using double emulsion (W/O/W) method and characterized by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Chitosan-PEG nanoparticles incorporated with the rabies whole attenuated virus antigen (CS-PEG NP-RV Ag.) were prepared using Ionic Gelation method. The CS-PEG NP-RV Ag. was surface modified with biocompatible polymers such as Acacia, Bovine Serum Albumin (BSA), Casein, Ovalbumin and Starch by Ionic Gelation method. The morphology was confirmed by SEM and Transmission Electron Microscopy (TEM). The surface modification was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), Zeta potential. The size distribution of CS-PEG-RV Ag. and surface modified CS-PEG-RV Ag. by respective biocompatible polymers was assessed by Zetasizer. Release profile of both stabilized nanoparticles was carried out by modified centrifugal ultrafiltration method which showed the sustained release pattern of the Rabies Ag. Immune stimulation under in-vitro condition was studied using rosette assay and phagocytosis assay. In-vitro toxicity using human blood and genotoxicity using human blood DNA was also studied to assess the toxicity of the nanoformulations. The results of these studies infer that PLGA-b-PEG nanoparticles, CS-PEG and surface modified CS-PEG nanoparticles may be an efficient nanocarrier for the RV Ag. to elicit immune response sustainably with negligible toxic effect to the human system. Graphical Figure options.
机译：纳米疫苗为以有效和持续的方式预防或治疗疾病引入了新的领域。各种聚合物已用于药物递送以增加治疗价值且副作用最小。因此，本研究结合了纳米技术和聚合物用于药物递送。采用双乳剂（W / O / W）方法将聚（d，l-乳酸-乙醇酸共聚物）-b-聚乙二醇与狂犬病全减毒病毒抗原掺入并通过扫描电子显微镜（SEM）表征和原子力显微镜（AFM）。使用离子凝胶法制备掺有狂犬病全减毒病毒抗原（CS-PEG NP-RV Ag。）的壳聚糖-PEG纳米颗粒。 CS-PEG NP-RV Ag。通过离子胶凝法用生物相容性聚合物（如阿拉伯胶，牛血清白蛋白（BSA），酪蛋白，卵清蛋白和淀粉）进行表面修饰。通过SEM和透射电子显微镜（TEM）确认了形态。表面改性通过傅立叶变换红外光谱法（FTIR），Zeta电位确认。 CS-PEG-RV Ag的尺寸分布。和表面改性的CS-PEG-RV Ag。通过各自的生物相容性聚合物的含量通过Zetasizer进行评估。两种稳定的纳米颗粒的释放曲线均通过改进的离心超滤方法进行，显示了狂犬病病毒的持续释放模式。使用玫瑰花结试验和吞噬作用试验研究了体外条件下的免疫刺激。还研究了使用人血的体外毒性和使用人血DNA的遗传毒性，以评估纳米制剂的毒性。这些研究的结果推断，PLGA-b-PEG纳米颗粒，CS-PEG和表面改性的CS-PEG纳米颗粒可能是RV Ag的有效纳米载体。持续引发免疫反应，对人体系统的毒性作用可忽略不计。图形选项。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号