...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Retrovirology >HIV-1 recombinants with multiple parental strains in low-prevalence, remote regions of Cameroon: Evolutionary relics?
【24h】

HIV-1 recombinants with multiple parental strains in low-prevalence, remote regions of Cameroon: Evolutionary relics?

机译：喀麦隆偏远地区低流行地区具有多个亲本菌株的HIV-1重组体：进化遗物？

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The HIV pandemic disseminated globally from Central West Africa, beginning in the second half of the twentieth century. To elucidate the virologic origins of the pandemic, a cross-sectional study was conducted of the genetic diversity of HIV-1 strains in villagers in 14 remote locations in Cameroon and in hospitalized and STI patients. DNA extracted from PBMC was PCR amplified from HIV(+) subjects. Partial pol amplicons (N = 164) and nearly full virus genomes (N = 78) were sequenced. Among the 3956 rural villagers studied, the prevalence of HIV infection was 4.9%; among the hospitalized and clinic patients, it was 8.6%. Results Virus genotypes fell into two distinctive groups. A majority of the genotyped strains (109/164) were the circulating recombinant form (CRF) known to be endemic in West Africa and Central West Africa, CRF02_AG. The second most common genetic form (9/164) was the recently described CRF22_01A1, and the rest were a collection of 4 different subtypes (A2, D, F2, G) and 6 different CRFs (-01, -11, -13, -18, -25, -37). Remarkably, 10.4% of HIV-1 genomes detected (17/164) were heretofore undescribed unique recombinant forms (URF) present in only a single person. Nearly full genome sequencing was completed for 78 of the viruses of interest. HIV genetic diversity was commonplace in rural villages: 12 villages each had at least one newly detected URF, and 9 villages had two or more. Conclusions These results show that while CRF02_AG dominated the HIV strains in the rural villages, the remainder of the viruses had tremendous genetic diversity. Between the trans-species transmission of SIVcpz and the dispersal of pandemic HIV-1, there was a time when we hypothesize that nascent HIV-1 was spreading, but only to a limited extent, recombining with other local HIV-1, creating a large variety of recombinants. When one of those recombinants began to spread widely (i.e. became epidemic), it was recognized as a subtype. We hypothesize that the viruses in these remote Cameroon villages may represent that pre-epidemic stage of viral evolution.
机译：背景技术从20世纪下半叶开始，艾滋病毒大流行从西非中部在全球传播。为了阐明大流行的病毒学起源，对喀麦隆14个偏远地区的村民以及住院和STI患者的HIV-1菌株的遗传多样性进行了横断面研究。从PBMC中提取的DNA从HIV（+）受试者中进行PCR扩增。对部分pol扩增子（N = 164）和几乎完整的病毒基因组（N = 78）进行了测序。在接受调查的3956个农村村民中，艾滋病毒感染率为4.9％;而艾滋病毒感染率为4.9％。在住院和门诊患者中，这一比例为8.6％。结果病毒基因型分为两个不同的组。大多数基因型菌株（109/164）是已知在西非和西非中部流行的循环重组形式（CRF），即CRF02_AG。第二种最常见的遗传形式（9/164）是最近描述的CRF22_01A1，其余的是4种不同亚型（A2，D，F2，G）和6种不同CRF（-01，-11，-13， -18，-25，-37）。值得注意的是，迄今检测到的10.4％的HIV-1基因组（17/164）是仅一个人存在的未描述的独特重组形式（URF）。 78种目标病毒的近全基因组测序已完成。 HIV遗传多样性在农村村庄很普遍：12个村庄每个都有至少一个新发现的URF，9个村庄有两个或更多个。结论这些结果表明，尽管CRF02_AG在农村地区的HIV菌株中占主导地位，但其余病毒具有巨大的遗传多样性。在SIVcpz的跨物种传播和大流行HIV-1的传播之间，有一段时间我们假设新生的HIV-1正在传播，但仅在有限的范围内与其他本地HIV-1重组，从而形成了一个大的病毒。多种重组体。当这些重组体之一开始广泛传播（即流行）时，它被认为是亚型。我们假设喀麦隆这些偏远村庄的病毒可能代表了病毒进化的流行前阶段。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号