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首页> 外文期刊>Research Opinions in Animal & Veterinary Sciences >Prevalence and some risk factors associated with brucellosis and leptospirosis in aborted fetuses of ruminant species
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Prevalence and some risk factors associated with brucellosis and leptospirosis in aborted fetuses of ruminant species

机译：反刍动物流产胎儿的布鲁氏菌病和钩端螺旋体病的患病率及一些危险因素

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In this study, a total of 91, 88, 82, 50 and 35 aborted bovine, ovine, caprine, buffalo and camel fetuses respectively were tested for the presence of Brucella spp. and Leptospira spp. From the total of 697 animal herds, 124 out of 220 bovine (56.36%), 102 out of 190 ovine (53.68%), 96 out of 165 caprine (58.18%), 48 out of 73 buffalo (65.75%) and finally 31 out 0f 49 camel (63.26%) dairy herds were infected by both Brucella spp. and Leptospira spp. In total 69 (19.94%) and 107 (30.92%) out of 346 aborted fetuses were positive for Brucella spp. and Leptospira spp. by culture methods. For the detection of Brucella and Leptospira species, the DNA was detected by multiplex PCR from 79 (22.83%) and 120 (34.68%) out of 346 aborted fetuses, respectively. Also, 32 (9.24%) of aborted fetuses were diagnosed positive for presences of both Brucella and Leptospira species by multiplex PCR. This result indicated that all of the 69 Brucella and 107 Leptospira culture-positive samples had also positive multiplex PCR and in addition to this the multiplex PCR detected Brucella spp in 10 samples and Leptospira spp. Therefore, our results showed the sensitivity and specificity of multiplex PCR assay (93 and 100% respectively). Our results indicated that the multiplex PCR assay was an accurate, sensitive, fast, safe and specific method for the detection of Brucella spp. and Leptospira spp. in aborted fetuses. Further, the results showed that caprine was the most susceptible and camel is the least susceptible species to the two infecting bacteria. In addition to above, our results showed that the most cases of abortion due to Brucella spp. and Leptospira spp. occurred in the spring. Furthermore, our results showed that abortion caused by Brucella spp. and Leptospira spp. occurred mostly in first partum followed by second, third, forth, fifth and the last partum.
机译：在这项研究中，分别测试了总共91、88、82、50和35个流产的牛，羊，山羊，水牛和骆驼胎儿的布鲁氏菌属。和钩端螺旋体在697个动物群中，220个牛中有124个（56.36％），190个绵羊中有102个（53.68％），165个山羊中有96个（58.18％），73个水牛中有48个（65.75％），最后是31个0f 49头骆驼（63.26％）的奶牛场都被布鲁氏菌属感染。和钩端螺旋体在346例流产胎儿中，布鲁氏菌属阳性的总数为69例（19.94％）和107例（30.92％）。和钩端螺旋体通过文化方法。为了检测布鲁氏菌和钩端螺旋体，通过多重PCR分别检测了346例流产胎儿中的79例（22.83％）和120例（34.68％）的DNA。另外，通过多重PCR诊断出32例（9.24％）流产胎儿的布鲁氏菌和钩端螺旋体均阳性。该结果表明，所有69个布鲁氏菌和107个钩端螺旋体培养阳性样品均具有阳性多重PCR，除此之外，该多重PCR还检测了10个样品中的布鲁氏菌属和钩端螺旋体。因此，我们的结果显示了多重PCR检测的灵敏度和特异性（分别为93％和100％）。我们的结果表明，多重PCR检测是检测布鲁氏菌属的准确，灵敏，快速，安全且特异的方法。和钩端螺旋体在流产的胎儿中。此外，结果表明，对于两种感染细菌，山羊皮最易感染，骆驼是最不易感染。除上述内容外，我们的结果还显示，大多数病例是由布鲁氏菌引起的流产。和钩端螺旋体发生在春天。此外，我们的结果表明流产是由布鲁氏菌引起的。和钩端螺旋体发生在第一部分，随后是第二，第三，第四，第五和最后一部分。

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