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首页> 外文期刊>Reumatologia >Serum IgG galactosylation in psoriatic arthritis patients undergoing a biological treatment. A preliminary report
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Serum IgG galactosylation in psoriatic arthritis patients undergoing a biological treatment. A preliminary report

机译：接受生物治疗的银屑病关节炎患者的血清IgG半乳糖基化。初步报告

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Aim of the study : A?group of psoriatic arthritis patients (PSA, 11 cases) has undergone a?treatment. For each patient two serum samples were collected: before the treatment (sample before) and after the treatment (sample after). Performed investigation aimed to provide an information whether a?treatment of the PSA patients improves the galactosylation of IgG N-glycans, which previously was shown for rheumatoid arthritis (RA) patients. Material and methods : Immunoglobulin G (IgG) was isolated from serum of PSA patients, before and after the treatment, using affinity chromatography on Protein A-Sepharose, and was further analyzed regarding the galactose content, using gas chromatography-mass spectrometry (GC-MS) method and ELISA test, performed with two lectins: Ricinus communis (RCA-I) and Griffonia simplicifolia (GSL-II). Based on ELISA results an agalactosylation factor (AF) was calculated for each before and after IgG sample. Results: Obtained data regarded galactose content in IgG, AF calculation and estimation of two laboratory indices of inflammation: erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) concentration in serum. Based on statistical methods it was shown that average difference (d) of after and before measures of AF for PSA patients was MedHL = –0.097 (95% CI: –0.49–0.25), which means that applied treatment did not affect AF value for this group of patients in a?statistically significant manner (p = 0.2936). Conclusions: The study demonstrated that, in the contrary to RA patients, IgG galactosylation in PSA patients, undergoing a?treatment, showed no statistically significant changes.
机译：研究目的：一组银屑病关节炎患者（PSA，11例）接受了治疗。对于每位患者，收集了两个血清样品：治疗前（治疗前）和治疗后（治疗后）。进行的研究旨在提供一种信息，对PSA患者进行治疗是否能改善IgG N-聚糖的半乳糖基化作用，以前曾对类风湿关节炎（RA）患者进行过研究。材料和方法：在治疗前后，使用蛋白A-Sepharose亲和层析从PSA患者血清中分离免疫球蛋白G（IgG），并使用气相色谱-质谱（GC-GC）进一步分析半乳糖含量MS）方法和ELISA测试，使用两种凝集素：Ricinus communis（RCA-I）和Griffonia simplicifolia（GSL-II）。根据ELISA结果，分别计算IgG样品之前和之后的半乳糖基化因子（AF）。结果：获得的数据涉及IgG中的半乳糖含量，AF的计算和两种炎症实验室指标的评估：血清中的红细胞沉降率（ESR）和C反应蛋白（CRP）浓度。根据统计学方法，PSA患者房颤测量前后的平均差异（d）为Med HL = –0.097（95％CI：–0.49–0.25），这意味着所应用的治疗并未以统计学上显着的方式影响该组患者的AF值（p = 0.2936）。结论：该研究表明，与RA患者相反，接受PS治疗的PSA患者IgG半乳糖基化没有统计学上的显着变化。

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