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首页> 外文期刊>Revista Brasileira de Biociências = >Spctrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide
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Spctrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide

机译：分光光度法测定丙酮，乙醇和二甲基亚砜中的叶绿体色素

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(Spctrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide). The concentration of pigment indicates a range of properties in the physiological processes of plants. A large number of procedures have been developed to measure leaf pigment content. Some of these procedures involve leaf maceration and centrifugation, while others require incubation of a leaf sample in solvent for a few minutes or hours, which makes the process laborious. The choice of the best procedure may vary according to the plant species and type of solvent used. This study compared the efficiency of three commonly used solvents (dimethylsulphoxide, ethanol and acetone) in the extraction of chlorophylls and carotenoids of five plant species and one lichen species. The results indicated that dimethylsulphoxide was more efficient at chlorophyll extraction in all of the studied species. The ethanol and acetone extracts quickly lost molecular stability. Acetone extract may also cause phaeophytinisation and reduce the chlorophyll content.
机译：分光光度法测定丙酮，乙醇和二甲基亚砜中的叶绿体色素）。色素的浓度表明了植物生理过程中的一系列特性。已经开发出许多程序来测量叶片色素含量。这些步骤中的一些涉及叶片浸渍和离心，而另一些则需要将叶片样品在溶剂中孵育几分钟或几小时，这使该过程费力。最佳方法的选择可能会根据植物种类和所用溶剂的类型而有所不同。这项研究比较了三种常用溶剂（二甲亚砜，乙醇和丙酮）在提取5种植物和1种地衣植物的叶绿素和类胡萝卜素中的效率。结果表明，在所有研究的物种中，二甲基亚砜在叶绿素提取方面更有效。乙醇和丙酮提取物很快失去了分子稳定性。丙酮提取物也可能引起植焦素化并降低叶绿素含量。

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