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首页> 外文期刊>Regenerative Therapy >Autologous and heterotopic transplantation of adipose stromal vascular fraction ameliorates stress urinary incontinence in rats with simulated childbirth trauma
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Autologous and heterotopic transplantation of adipose stromal vascular fraction ameliorates stress urinary incontinence in rats with simulated childbirth trauma

机译：脂肪间质血管部分的自体和异位移植改善了模拟分娩创伤大鼠的压力性尿失禁

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Introduction Autologous transplantation of adipose stromal vascular fraction (SVF) is a cost-effective and technically accessible option for cell therapy. Clinical study of SVF transplantation for male stress urinary incontinence (SUI) is underway, but the effectiveness remains unknown for female SUI, majority of which is caused by childbirth trauma. Methods Vaginal Distension (VD) rats were generated as in?vivo model for female SUI. To quantitate the severity of SUI, leak point pressure (LPP) was measured by placing a bladder catheter. There was a characteristic waveform of LPP with two-peaks, and we counted the second peak as an LPP value. Adipose SVF was separated from inguinal fat and delivered into external urethral sphincter (EUS) through transperineal injection. LPP was measured 7 or 14 days after SVF transplantation. Tissue damage and collagen synthesis around the EUS were visualized by Masson's trichrome and eosin staining. Antibody against α-smooth muscle actin (α-SMA) was used to stain smooth muscle or activated stromal cells. Donor SVF cells were distinguished from recipient EUS tissue by tracking with GFP transgene. Results VD procedure decreased the frequency at which the normal LPP waveform appeared and lowered the LPP value. SVF injection normalized the waveform as well as the level of LPP. VD disrupted histological structure of EUS and SVF failed to differentiate into striatal muscles. Instead, SVF increased α-SMA positive cells and collagen synthesis but the phenomena depended on VD stimulus. GFP tracking indicated that the transplanted SVF cells persisted for four weeks and synthesized α-SMA protein simultaneously. Conclusions Autologous transplantation of adipose SVF displayed bulking effects through collagen synthesis. However, such heterotopic activation was dependent on tissue damage.
机译：简介脂肪间质血管部分（SVF）的自体移植是细胞治疗的一种经济高效且技术上可行的选择。 SVF移植治疗男性压力性尿失禁（SUI）的临床研究正在进行中，但女性SUI的有效性尚不明确，其中大部分是由分娩创伤引起的。方法以雌性SUI的体内模型制作阴道扩张（VD）大鼠。为了量化SUI的严重程度，通过放置膀胱导管来测量泄漏点压力（LPP）。有一个具有两个峰值的LPP的特征波形，我们将第二个峰值算作LPP值。从腹股沟脂肪中分离出SVF脂肪，并通过会阴注射将其输送到外尿道括约肌（EUS）中。 SVF移植后7或14天测量LPP。通过Masson的三色和曙红染色可以观察到EUS周围的组织损伤和胶原蛋白合成。抗α-平滑肌肌动蛋白（α-SMA）抗体用于染色平滑肌或活化的基质细胞。通过追踪GFP转基因，将供体SVF细胞与受体EUS组织区分开。结果VD程序降低了正常LPP波形出现的频率并降低了LPP值。 SVF注入对波形以及LPP的电平进行归一化。 VD破坏了EUS和SVF的组织学结构，未能分化为纹状肌。相反，SVF增加了α-SMA阳性细胞和胶原蛋白的合成，但这种现象取决于VD刺激。 GFP追踪表明，移植的SVF细胞持续了四个星期，并同时合成了α-SMA蛋白。结论脂肪SVF的自体移植通过胶原合成表现出膨松作用。但是，这种异位激活取决于组织损伤。

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