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首页> 外文期刊>Regenerative Therapy >Original Article Osteogenic differentiation enhances the MC3T3-E1 secretion of glycosaminoglycans with an affinity for basic fibroblast growth factor and bone morphogenetic protein-2
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Original Article Osteogenic differentiation enhances the MC3T3-E1 secretion of glycosaminoglycans with an affinity for basic fibroblast growth factor and bone morphogenetic protein-2

机译：成骨细胞分化增强糖胺聚糖的MC3T3-E1分泌，对碱性成纤维细胞生长因子和骨形态发生蛋白2具有亲和力

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Introduction It is generally recognized that a wide variety of morphogens and growth factors bind to the glycosaminoglycans (GAG) of proteoglycans (PG) to affect their bioavailability to ligands. Many growth factors involving in osteogenic differentiation require the GAG side chains to facilitate their interaction to the cell surface receptors and the biosynthesis of osteogenic proteins. The objective of this study is to investigate the secretion of GAG from MC3T3-E1 pre-osteoblasts of a murine bone calvaria during the osteogenic differentiation. Methods When MC3T3-E1 cells were cultured in the differentiation medium (DM) containing bone morphogenetic protein (BMP)-2, the alkaline phosphatase activity, calcium content and the amount of basic fibroblast growth factor (bFGF)- or BMP-2-bound sulfated GAG were determined. Moreover, the disaccharide analysis of the GAG was performed. Results When MC3T3-E1 cells were cultured in the differentiation medium (DM) containing bone morphogenetic protein (BMP)-2, the alkaline phosphatase activity and calcium content were significantly enhanced compared with those of the BMP-2-free DM and normal medium with or without BMP-2. Significantly higher amount of GAG secreted was detected for cells cultured in the DM containing BMP-2, in contrast to other culture conditions. The GAG secreted had an affinity for BMP-2 and basic fibroblast growth factor (bFGF). The disaccharide analysis of GAG demonstrated that the percentage of ΔHexA α1,4GlcNSO3 and ΔHexA (2-OSO3) α1,4GlcNSO3 increased, but that of ΔHexA α1,4GlcNSO3(6-OSO3) decreased (ΔHexA: unsaturated uronic acid residue, GlcNSO3: N-sulfated glucosamine, ΔHexA (2-OSO3): unsaturated uronic acid 2-sulfate residue, GlcNSO3(6-OSO3): N-sulfated glucosamine 6-sulfated). Conclusion It was found that the osteogenic differentiation allowed cells to enhance the secretion of GAG with an affinity for BMP-2 and bFGF.
机译：引言一般认为，各种各样的形态发生子和生长因子与蛋白聚糖（PG）的糖胺聚糖（GAG）结合，影响它们对配体的生物利用度。许多涉及成骨分化的生长因子都需要GAG侧链来促进它们与细胞表面受体的相互作用以及成骨蛋白的生物合成。这项研究的目的是调查成骨分化过程中小鼠骨颅的MC3T3-E1前成骨细胞中GAG的分泌。方法在含有骨形态发生蛋白（BMP）-2的分化培养基（DM）中培养MC3T3-E1细胞时，碱性磷酸酶活性，钙含量和碱性成纤维细胞生长因子（bFGF）或BMP-2结合测定了硫酸化的GAG。此外，进行了GAG的二糖分析。结果在含有骨形态发生蛋白（BMP）-2的分化培养基（DM）中培养MC3T3-E1细胞时，与不含BMP-2的DM和普通培养基相比，碱性磷酸酶活性和钙含量显着提高。或没有BMP-2。与其他培养条件相比，在含有BMP-2的DM中培养的细胞中检测到的GAG分泌量明显更高。分泌的GAG对BMP-2和碱性成纤维细胞生长因子（bFGF）具有亲和力。 GAG的二糖分析表明，ΔHexAα1,4GlcNSO 3 和ΔHexA（2-OSO 3 ）α1,4GlcNSO 3 的百分比增加，但ΔHexAα1,4GlcNSO 3 （6-OSO 3 ）的下降（ΔHexA：不饱和糖醛酸残基，GlcNSO 3 ：N-硫酸化氨基葡萄糖，ΔHexA（2-OSO 3 ）：不饱和糖醛酸2-硫酸酯残基，GlcNSO 3 （6-OSO 3 ）：N-硫酸化的氨基葡萄糖（6-硫酸化）。结论发现成骨细胞分化可以使细胞增强GAG的分泌，并具有对BMP-2和bFGF的亲和力。

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