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首页> 外文期刊>Regenerative Therapy >Electron microscopic observation of human auricular chondrocytes transplanted into peritoneal cavity of nude mice for cartilage regeneration
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Electron microscopic observation of human auricular chondrocytes transplanted into peritoneal cavity of nude mice for cartilage regeneration

机译：人耳软骨细胞移植入裸鼠腹腔软骨再生的电镜观察

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Restoration of damaged cartilage tissue has been deemed futile with current treatments. Although there have been many studies on cartilage regeneration thus far, there is no report that chondrocytes were completely re-differentiated in?vitro. The clarification of cellular composition and matrix production during cartilage regeneration must be elucidated to fabricate viable mature cartilage in?vitro. In order to achieve this aim, the chondrocytes cultured on coverslips were transplanted into the peritoneal cavities of mice. At different time points post-transplantation, the cartilage maturation progression and cells composing the regeneration were examined. Cartilage regeneration was confirmed by hematoxylin & eosin (HE) and toluidine blue staining. The maturation progression was carefully examined further by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). At the first and second week time points, various cell shapes were observed using SEM. Chronologically, by the third week, the number of fibers increased, suggesting the progression of extracellular matrix (ECM) maturation. Observation through TEM revealed the chondrocytes located in close proximity to various cells including macrophage-like cells. On the second week, infiltration of lymphocytes and capillary vessels were observed, and after the third week, the chondrocytes had matured and were abundantly releasing extracellular matrix. Chronological observation of transplanted chondrocytes by electron microscopy revealed maturation of chondrocytes and accumulation of matrix during the re-differentiation process. Emerging patterns of host-derived cells such as macrophage-like cells and subsequent appearance of lymphocytes-like cells and angiogenesis were documented, providing crucial context for the identification of the cells responsible for in?vivo cartilage regeneration.
机译：在目前的治疗中，受损软骨组织的恢复被认为是徒劳的。尽管迄今为止有许多关于软骨再生的研究，但是没有报道软骨细胞在体外完全重新分化。必须阐明在软骨再生过程中细胞组成和基质产生的澄清，以体外制备可行的成熟软骨。为了达到这个目的，将在盖玻片上培养的软骨细胞移植到小鼠的腹膜腔中。在移植后的不同时间点，检查软骨成熟进程和组成再生的细胞。苏木精和曙红（HE）和甲苯胺蓝染色证实了软骨的再生。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）进一步仔细检查了成熟过程。在第一和第二周时间点，使用SEM观察到各种细胞形状。按时间顺序，到第三周，纤维数量增加，表明细胞外基质（ECM）成熟的进程。通过TEM观察发现，软骨细胞位于包括巨噬细胞样细胞在内的各种细胞的附近。在第二周，观察到淋巴细胞和毛细血管的浸润，第三周后，软骨细胞已经成熟并大量释放细胞外基质。通过电子显微镜对移植的软骨细胞进行时间序列观察，发现在再分化过程中软骨细胞的成熟和基质的积累。记录了宿主衍生细胞（例如巨噬细胞样细胞）的新兴模式以及随后出现的淋巴细胞样细胞和血管生成，为鉴定负责体内软骨再生的细胞提供了重要背景。

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