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首页> 外文期刊>Regenerative Therapy >Original Article Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates
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Original Article Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates

机译：足够的泰勒库埃特流介导的剪切应力可用于分离人iPS细胞来源的细胞聚集体

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摘要

Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.
机译：包括诱导多能干细胞（iPSC）在内的多能干细胞是用于再生医学和三维悬浮培养技术的有前途的细胞来源，这些技术可以通过细胞聚集产生大量所需细胞。尽管手动程序被广泛用于解离细胞聚集体，但使用设备开发非手动程序将有助于有效的细胞生产。在本研究中，我们开发了新型的细胞聚集体解离装置，该装置具有基于Taylor couette流介导的剪切应力的旋转圆柱体。可以根据装置内部的旋转筒的尺寸和转速的增加来增加剪切应力。在人iPSC的未分化扩增和心脏分化后，足够的器械尺寸和合适的旋转速率可有效地解离细胞聚集体。这些发现表明非手动装置程序​​可能对从人iPSC衍生的细胞聚集体中收获单个细胞有用。

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