Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function

Naoki Momotori; Jun-ichiro Jo; Yasuhiko Tabata

首页> 外文期刊>Regenerative Therapy >Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function

【24h】

Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function

机译：能够释放吡格列酮以修饰巨噬细胞功能的聚合物微球的制备

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Introduction Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology. Method In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In?vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1?wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)?10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated. Results The sustained release of pioglitazone was observed from all types of pio-MS in?vitro. When BMDM were cultured with the pio-MS with an average diameter of 40?μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS. Conclusions The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function.

机译：简介巨噬细胞在调节炎症和组织再生中起重要作用。已知抗炎性巨噬细胞在组织再生中起重要作用。这项研究的目的是通过利用药物递送技术来改变巨噬细胞表型的抗炎功能。方法在本研究中，制备了4种类型的聚（L-乳酸-乙醇酸共聚物）（PLGA）微球，其中掺入了不同大小的抗炎修饰剂吡格列酮（pio-MS）。 pio-MS的体外释放试验是在含有1wt％月桂基硫酸钠的磷酸盐缓冲盐溶液（PBS）中进行的。评价了用pio-MS培养的小鼠骨髓衍生巨噬细胞（BMDM）的精氨酸酶活性和白细胞介素（IL）α10作为抗炎巨噬细胞标记物的分泌。结果从各种类型的体外pio-MS观察到吡格列酮的持续释放。用平均直径为40？μm的pio-MS（pio-MS40）培养BMDM时，与其他pio-MS相比，精氨酸酶活性和IL-10分泌显着增加。结论直径40μm的pio-MS40具有诱导BMDM抗炎修饰的潜力。吡格列酮的持续释放促进了巨噬细胞功能的改变。

著录项

来源
《Regenerative Therapy》 |2019年第1期|共8页
作者
Naoki Momotori; Jun-ichiro Jo; Yasuhiko Tabata;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类临床医学;
关键词
MacrophagesPioglitazoneDrug delivery systemPoly(L-lactic-co-glycolic acid)PLGAMicrospheresPLGA poly(L-lactic-co-glycolic acid)pio-MS PLGA microspheres incorporating pioglitazonePBS phosphate buffered-saline solutionIL interleukinBMDM mouse bone marrow derived-macrophageiNOS inducible nitric oxide synthaseTNF tumor necrosis factorPPARγ peroxisome proliferator-activated receptor γPLA poly( l -lactic acid)PGA poly(glycolic acid)DDW double-distilled waterRS resulting solutionSEM scanning electron microscopyHPLC high performance liquid chromatographyUV ultra violetIMDM Iscove's modified Dulbecco's mediumFBS fetal bovine serumM-CSF macrophage colony stimulating factorELISA enzyme-linked immunosorbent assayPCR polymerase chain reactionSD standard deviation;

机译：巨噬细胞吡格列酮药物递送系统聚（L-乳酸-乙醇酸）PLGA微球PLGA聚（L-乳酸-乙醇酸）pio-MS PLGA微球并入吡格列酮PBS磷酸缓冲盐溶液IL白介素BMDM小鼠骨髓衍生的巨噬细胞NOS诱导型一氧化氮合酶因子PPARγ过氧化物酶体增殖物激活受体γPLA聚（1-乳酸）PGA聚（乙醇酸）DDW双蒸馏水RS生成溶液SEM扫描电镜HPLC高效液相色谱UV紫外线IMDM Iscove修饰的Dulbecco培养基FBS胎牛血清M-CSF巨噬细胞集落刺激酶联免疫吸附法PCR聚合酶链反应SD标准差;

相似文献

外文文献
中文文献
专利

1. Preparation and evaluation of modified release ibuprofen microspheres with acrylic polymers (Eudragit) by quasi-emulsion solvent diffusion method: effect of variables. [J] . Devrim B, Canefe K Acta Poloniae Pharmaceutica: Durg Research . 2006,第6期

机译：准乳液溶剂扩散法制备丙烯酸酯类聚合物布洛芬微球及其评价：变量的影响。
2. Polyorganophosphazene microspheres for drug release: polymer synthesis, microsphere preparation, in vitro and in vivo naproxen release [J] . Veronese FM., Caliceti P., De Filippis P., Journal of Controlled Release: Official Journal of the Controlled Release Society . 1998,第3期

机译：用于药物释放的聚有机磷腈微球：聚合物合成，微球制备，萘普生的体内和体外释放
3. Preparation of Controlled Releasing Acrylic Polymer Microspheres of Acebutolol Hydrochloride and Those Powder Coated Microspheres with Sodium Alginate in a Polymeric Spherical Crystallization System [J] . Fude Cui, Yoshiaki Kawashima, Hirofumi Takeuchi, Chemical and Pharmaceutical Bulletin . 1996,第4期

机译：聚合物球形结晶体系中盐酸醋丁酚可控释放丙烯酸聚合物微球的制备及海藻酸钠粉末包覆微球的制备
4. Preparation of Raspberry Ketone Modified Chitosan Microspheres and Effect on Macrophage Activation [C] . Gregory S. McGraw, Alex Hoban, Daniel Abebe, Society for Biomaterials annual meeting and exposition . 2014

机译：覆盆子酮修饰的壳聚糖微球的制备及其对巨噬细胞活化的影响
5. Design, preparation and characterization of modified release oral drug delivery systems based on hydrophilic polymer matrices. [D] . El-Malah, Yasser M. 2007

机译：基于亲水性聚合物基质的调释口服药物递送系统的设计，制备和表征。
6. Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function [O] . Naoki Momotori, Jun-ichiro Jo, Yasuhiko Tabata 2019

机译：能够释放吡格列酮以修饰巨噬细胞功能的聚合物微球的制备
7. Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function [O] . Naoki Momotori, Jun-ichiro Jo, Yasuhiko Tabata 2019

机译：高分子微球的制备能够释放吡格列酮释放以改变巨噬细胞功能

Preparation of polymer microspheres capable for pioglitazone release to modify macrophages function

摘要

著录项

相似文献

相关主题

期刊订阅