Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

Alan G. Gon?alves; Diogo R. B. Ducatti; Franciely G. Colodi; M. Eugênia R. Duarte; Miguel D. Noseda

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Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

机译：通过半乳聚糖的部分酸水解生产琼脂糖和卡拉寡糖

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Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 oC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.

机译：通过商业琼脂糖和κ-角叉菜胶的部分酸水解产生了Agaro-和carra-寡糖。通过凝胶过滤色谱法纯化二糖和四糖，并通过NMR（1D和2D）光谱和ESIMS进行表征。获得了以下寡糖：琼脂二糖，琼脂四糖，κ-卡拉比糖和κ-卡拉酸酯。以琼脂二糖和琼脂四糖为标准，开发了一种高性能尺寸排阻色谱法（HPSEC），该方法用于研究琼脂糖的水解速率和寡糖的生产。水解六个小时（0.1 M TFA，65 oC）主要产生二糖和四糖。在本工作中开发的寡糖生产和评估方法论显示了生产生物活性寡糖的良好潜力。

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《Revista Brasileira de Farmacognosia》 |2011年第2期|共9页
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Alan G. Gon?alves; Diogo R. B. Ducatti; Franciely G. Colodi; M. Eugênia R. Duarte; Miguel D. Noseda;
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1. The Isolation of O-β-d-Galactopyranosyl-(1→14)-d-galactose: a Product of the Partial Acid Hydrolysis of the Galactan of White-Lupin (Lupinus albus) Seeds [J] . G. M. Gray, J. B. Hay The biochemical journal . 1966,第3期

机译：O-β-d-半乳糖吡喃糖基-（1→14）-d-半乳糖的分离：白羽扇豆种子半乳聚糖部分酸水解的产物
2. Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method [J] . LI Guangsheng, ZHAO Xia, LV Youjing, 中国海洋大学学报（英文版） . 2015,第002期

机译：微波辅助酸水解法制备κ-卡拉寡糖
3. Selective Hydrolysis of the 3,6-Anhydrogalacotosidie Linkage in Red Algal Galactan: A Combination of Reductive Acid Hydrolysis and Anhydrous Mercaptolysis [J] . Yoichiro Hama, Aya Tsuneoka, Ryoji Morita Bioscience, Biotechnology, and Biochemistry . 2010,第9期

机译：红色藻半乳聚糖中3,6-脱水半乳糖苷键的选择性水解：还原酸水解和无水巯基水解的组合。
4. Production of Upgraded Bio-oils from Miscanthus Acid Hydrolysis Residues by Catalytic Pyrolysis – Effect of Feed and Acid Hydrolysis Conditions [C] . K. Kalogiannis, S. Stefanidis, C. Michailof, European biomass conference and exhibition . 2012

机译：芒草酸水解残渣催化热解提纯生物油的研究-进料和酸水解条件的影响
5. PARTIAL ACID HYDROLYSIS PRETREATMENT FOR ENZYMATIC HYDROLYSIS OF CELLULOSE: A PROCESS DEVELOPMENT STUDY FOR ETHANOL PRODUCTION. [D] . KNAPPERT, DIANE RUTH. 1981

机译：纤维素酶水解的部分酸水解预处理：生产乙醇的工艺开发研究。
6. The isolation of O-beta-D-galactopyranosyl-(1 to 4)-D-galactose: a product of the partial acid hydrolysis of the galactan of white-lupin (Lupinus albus) seeds. [O] . J B Hay, G M Gray 1966

机译：O-β-D-半乳糖吡喃糖基-（1-4）-D-半乳糖的分离：白色羽扇豆（羽扇豆）种子半乳聚糖的部分酸水解产物。
7. Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans [O] . Diogo R. B. Ducatti, Franciely G. Colodi, Alan G. Gonçalves, 2011

机译：通过半乳聚糖的部分酸水解生产琼脂和低聚糖

Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans

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