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首页> 外文期刊>Revista Brasileira de Farmacognosia >Effects of heavy metals and light levels on the biosynthesis of carotenoids and fatty acids in the macroalgae Gracilaria tenuistipitata (var. liui Zhang & Xia)
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Effects of heavy metals and light levels on the biosynthesis of carotenoids and fatty acids in the macroalgae Gracilaria tenuistipitata (var. liui Zhang & Xia)

机译:重金属和轻水平对大型藻类Gracilaria tenuistipitata(var。liui Zhang&Xia)中类胡萝卜素和脂肪酸生物合成的影响

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We present here the effect of heavy metals and of different light intensities on the biosynthesis of fatty acids and pigments in the macroalga Gracilaria tenuistipitata (var. liui Zhang & Xia). In order to verify the fatty acid content, gas chromatography with flame ionization detection (GC-FID) was employed. Pigments (major carotenoids and chlorophyl-a) were monitored by liquid chromatography with diode array detection (HPLC-DAD). Cultures of G. tenuistipitata were exposed to cadmium (Cd2+, 200 ppb) and copper (Cu2+, 200 ppb), as well as to different light conditions (low light: 100 μmol.photons.m-2.s-1, or high light: 1000 μmol.photons.m-2.s-1). Cd2+ and Cu2+ increased the saturated and monounsaturated fatty acid content [14:0, 16:0, 18:0, 18:1 (n-7) and 18:1 (n-9)] and all major pigments (violaxanthin, antheraxanthin, lutein, zeaxanthin, chlorophyll-a and β-carotene). Both heavy metals decreased the levels of polyunsaturated fatty acids (PUFA) [18:2 (n-6), 18:3 (n-6), 18:5 (n-4), 20:4 (n-6), 20:5 (n-3), 22:6 (n-3)]. G. tenuistipitata cultures were exposed to high light intensity for five days and no statistically significant differences were observed in the content of fatty acids. On the other hand, the levels of pigments rose markedly for chlorophyll-a and all of the carotenoids studied.
机译:在这里,我们介绍重金属和不同光强度对大型藻类龙须菜(var。liui Zhang&Xia)中脂肪酸和色素的生物合成的影响。为了验证脂肪酸含量,采用了带有火焰电离检测的气相色谱仪(GC-FID)。通过液相色谱和二极管阵列检测(HPLC-DAD)监测颜料(主要类胡萝卜素和叶绿素-a)。 G. tenuistipitata的培养物暴露于镉(Cd2 +,200 ppb)和铜(Cu2 +,200 ppb)以及不同的光照条件下(弱光:100μmol。光子.m-2.s-1或高光:1000μmol。光子.m-2.s-1)。 Cd2 +和Cu2 +增加了饱和和单不饱和脂肪酸的含量[14:0、16:0、18:0、18:1(n-7)和18:1(n-9)]和所有主要色素(紫黄质,花药黄质) ,叶黄素,玉米黄质,叶绿素a和β-胡萝卜素)。两种重金属均降低了多不饱和脂肪酸(PUFA)的水平[18:2(n-6),18:3(n-6),18:5(n-4),20:4(n-6), 20:5(n-3),22:6(n-3)]。 G. tenuistipitata培养物暴露于高光强度下五天,脂肪酸含量未见统计学显着差异。另一方面,叶绿素-a和所有研究的类胡萝卜素的色素含量均显着上升。

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