...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista Brasileira de Fruticultura >Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)
【24h】

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

机译：分子标记在金针菇遗传变异分析中的应用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acerola (Malpighia emarginata) is a tropical fruit native from Central America and north of South America. It has shown an increasing economic and social importance due to its high vitamin C (ascorbic acid) content. Vegetative propagation is the preferable method used to establish acerola plantations. However, propagation by seeds has also been used allowing the identification and selection of genotypes that carry characteristics of agronomic interest. Twenty-four acerola accesses, of the Active Germplasm Bank of the Universidade Estadual de Londrina, were analyzed using molecular markers obtained with random amplified polymorphic DNA (RAPD) and simple sequence repeated (SSRs) primers. A total of 164 and 73 markers were obtained with RAPD and SSR primers, respectively. Polymorfic markers were scored as present or absent and analyzed using the UPGMA cluster analysis. The results presented reveal high levels of polymorphism in the studied collection. Comparative analysis of the phenograms, generated with both RAPD and SSR primers, revealed that while some accesses clustered in different groups other accesses presented the same association. However, there was large RAPD variation among the accesses. The associations observed with molecular markers were, for many accesses, the same of those determined on the basis of morphological characters.
机译：Acerola（Malpighia emarginata）是一种热带水果，产自中美洲和南美洲北部。由于其高的维生素C（抗坏血酸）含量，它已显示出越来越高的经济和社会重要性。营养繁殖是建立针叶树人工林的首选方法。但是，也已使用种子繁殖，以鉴定和选择具有农艺学特征的基因型。使用随机扩增的多态性DNA（RAPD）和简单重复序列（SSR）引物获得的分子标记，分析了隆德里纳国立大学活动种质库的二十四个针叶树的通道。用RAPD和SSR引物分别获得了164和73个标记。将多分子标记记为存在或不存在，并使用UPGMA聚类分析进行分析。呈现的结果揭示了所研究集合中的高水平多态性。通过RAPD和SSR引物生成的字符图的比较分析表明，尽管某些访问聚集在不同的组中，但其他访问却呈现相同的关联。但是，访问之间的RAPD差异很大。在许多访问中，观察到的与分子标记物的关联与基于形态特征确定的关联相同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号