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首页> 外文期刊>Revista Brasileira de Fruticultura >Avalia??o de protocolo para obten??o de mudas micropropagadas de bananeira cv. Prata-An? (subgrupo AAB)
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Avalia??o de protocolo para obten??o de mudas micropropagadas de bananeira cv. Prata-An? (subgrupo AAB)

机译：协议评估以获得微繁的香蕉幼苗cv。银安？ （AAB子组）

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摘要

The purpose of this work was to evaluate a micropropagation system to cultivar Prata-An?, observing the main factors of efficiency and limitation in the plantlet production process. The explants were established in the medium MS, supplemented with 5mg/L BAP (benzilaminopurine), 30g/L sucrose, 8g/L agar and pH 5.7. The bud multiplication was carried out in the same medium described above and the plantlet rooting phase was done with half concentration of salt and sucrose, without plant growth regulator. Losses by bacterial contamination were higher during the in vitro establishment and by fungus in the last two subcultures. The highest multiplication rates occurred between the fourth and the fifth subcultures and at the end of the process it was observed higher proportion of plantlets between 30 and 60mm. In relation to the efficiency of the number of glasses processed per operator in the laboratory it was low in the establishment, phase first subculture and rooting, when compared with the exponential multiplication phase of the explants. Even though it was noticed losses in the acclimatization phase, it was observed the occurrence of 1% of plantlets with morphological variation.
机译：这项工作的目的是评估一种针对Prata-An？品种的微繁系统，并观察其在苗生产过程中效率和限制的主要因素。外植体在添加5mg / L BAP（苯甲氨基嘌呤），30g / L蔗糖，8g / L琼脂和pH 5.7的MS培养基中建立。在与上述相同的培养基中进行芽繁殖，并在无植物生长调节剂的情况下，以一半浓度的​​盐和蔗糖进行小苗生根阶段。在体外建立过程中，细菌污染造成的损失较高，而在最近两次传代培养中，真菌造成的损失较高。最高的繁殖率发生在第四次和第五次传代之间，在该过程结束时，观察到30mm和60mm之间的幼苗比例更高。在实验室中，每个操作员处理的玻璃杯数量与外植体的指数倍增阶段相比，建立，第一阶段传代培养和生根的效率较低。即使在适应阶段发现损失，但观察到有1％的幼苗发生形态变化。

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