...
首页> 外文期刊>Revista Brasileira de Fruticultura >Potencial organogenético de tecidos caulinares e radiculares de caquizeiro
【24h】

Potencial organogenético de tecidos caulinares e radiculares de caquizeiro

机译:柿茎和根组织的器官发生潜能

获取原文
   

获取外文期刊封面封底 >>

       

摘要

With the objective of studying the morfogenesis in stems and root tissue of japanese persimmon, several experiments were conducted in the field and in vitro. The field experiments consisted of a series of works that involved the cutting of the stem and of the root, the layer and the air-layer of the japanese persimmon ' Fuyu'. In the cutting of the stem, the number of leaves, concentrations of IBA, the located and the total etiolation were tested. In the cutting of the root, the diameter and the position of the stake were tested. The layer was tested in the field and in recipients, being combined with the etiolation of the shoots, ring and application of IBA. In the air-layer the ring and the application of IBA were tested, being the air-layers used for another test with IBA. The experiments in vitro were conducted with juvenile plants, being a works accomplished for the organogenesis induction starting from root segments with BAP, TDZ and cinetina (10μM), combined with IAA (0,01μM), and another for the induction of the budding rooting for the permanence culture medium with 25mg.L-1 of IBA and latter transfer for another exempt culture medium of growth regulators and with activated coal. In all the experiments of cutting, layer and air-layer didn't occur the formation of roots. Just in the root cutting there was organogenesis, where the best budding and rooting responses were obtained with the stakes of larger diameter and in the vertical position. The organogenesis was also observed in root segments in vitro, with a great proliferation of callus and buds induced by the use of TDZ plus IAA. Unlike observed in the field, the in vitro budding took root with a rate of 66%, with a medium emission of 4,7 roots per shoot, being a promising technique to the cloning of the japanese persimmon.
机译:为了研究柿饼茎和根组织中的致畸作用,在野外和体外进行了一些实验。田间试验由一系列工作组成,涉及日本柿子“扶余”的茎和根的切割,层和气层的切割。在茎的切割中,测试了叶子的数量,IBA的浓度,位置和总黄化。在切割根部时,测试了木桩的直径和位置。该层在野外和接受者中进行了测试,并与嫩芽的生黄,环生和IBA的应用相结合。在空气层中,对环和IBA的应用进行了测试,这是用于IBA的另一项测试的空气层。体外实验是在幼年植物上进行的,是从BAP,TDZ和cinetina(10μM)的根段与IAA(0,01μM)结合开始的器官发生诱导工作,以及另一种诱导出芽生根的工作。含有25mg.L-1 IBA的永久性培养基,然后转移至另一种生长调节剂豁免培养基和活性炭中。在所有的切割实验中,都没有形成层和气层。仅在根部切割中就有器官发生,在较大直径和垂直位置的木桩中可获得最佳的出芽和生根响应。在体外的根节中也观察到器官发生,通过使用TDZ加IAA诱导愈伤组织和芽的大量增殖。与在田间观察到的不同,离体出芽以66%的比率生根,每个芽中散发4.7个根,这是克隆日本柿子的有前途的技术。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号