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首页> 外文期刊>Revista Brasileira de Fruticultura >Desenvolvimento in vitro de plantulas de diplóides de bananeira obtidas a partir de cultura de embri?es
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Desenvolvimento in vitro de plantulas de diplóides de bananeira obtidas a partir de cultura de embri?es

机译：胚胎培养获得的香蕉二倍体幼苗的体外发育

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摘要

The objective of this work was to evaluate the development of in vitro plantlets of progenies of eight diploid banana genotypes that were obtained from embryo culture. The genotypes were the wild species Calcutta and Malaccensis (Musa acuminata-AA), Butuhan and Fran?a (M. balbisiana-BB) and the hybrids 0304-02, 1304-06, 4252-04 and 9379-09 (M. acuminata-AA). The embryos were aseptically, being introduced in MS medium with 30 g L-1 agar, initially in Petri dishes (40 days) and afterwards in test tubes (45 days). It was observed a pronounced effect of the genotype on the in vitro development of the embryos. The progenies of the wild genotypes of the BB group, followed by that of AA hybrids showed higher development for the studied variables. The protocol was appropriated for the embryo culture of progenies of the eight genotypes. However, in order to have a non accentuated rooting, the period of in vitro development should be reduced to 30 days.
机译：这项工作的目的是评估从胚胎培养获得的八种二倍体香蕉基因型后代的体外小植株的发育。基因型是野生物种加尔各答和马六甲（Musa acuminata-AA），布图汉和Fran？a（M. balbisiana-BB）以及杂种0304-02、1304-06、4252-04和9379-09（M. acuminata） -AA）。无菌地将胚放入含有30 g L-1琼脂的MS培养基中，最初在培养皿中（40天），然后在试管中（45天）。观察到基因型对胚胎体外发育的显着影响。 BB组的野生基因型的后代，然后是AA杂种的后代显示出更高的发展为研究变量。该协议适用于八种基因型后代的胚胎培养。但是，为了不生根，体外发育期应缩短至30天。

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