Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis

Marcia D. Wigg; Maria J. Andrada-serpa; Maria T. V. Romanos; Mariana S. Pereira; Norma Santos; Paulo A. S. Mour?o; Yocie Yoneshigue-valentin

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Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis

机译：来自海藻的硫酸盐岩藻聚糖通过不含HTLV-1的颗粒抑制HeLa细胞感染：半定量分析

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A sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nested-polymerase chain reaction (PCR). The sulfated polysaccharide was not toxic to the cells at a concentration of 100 μg/mL and prevented infection by the viral particles when added to the cell monolayers. The proviral cDNA was only detected when the sulfated polysaccharide was added to the cells three hours post-infection, indicating that the inhibitory activity occurred in the initial stages of virus-cell interaction. Our results demonstrate, for the first time, the ability of a sulfated fucan from marine algae to inhibit virus transmission through free virus particles.

机译：来自海带海藻的硫酸盐岩藻聚糖阻止了从MT-2细胞系纯化的HTLV-1颗粒与HeLa细胞的相互作用。仅通过通过巢式聚合酶链反应（PCR）扩增新合成的HTLV-1前病毒cDNA来检测使用浓缩病毒悬浮液获得的感染。硫酸化多糖以100μg/ mL的浓度对细胞无毒，当添加到细胞单层时，可防止病毒颗粒感染。仅在感染后三小时将硫酸化多糖添加到细胞中时，才检测到前病毒cDNA，这表明抑制活性发生在病毒-细胞相互作用的初始阶段。我们的结果首次证明了海藻中的硫酸化岩藻聚糖抑制病毒通过自由病毒颗粒传播的能力。

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《Revista Brasileira de Farmacognosia》 |2011年第2期|共5页
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Marcia D. Wigg; Maria J. Andrada-serpa; Maria T. V. Romanos; Mariana S. Pereira; Norma Santos; Paulo A. S. Mour?o; Yocie Yoneshigue-valentin;
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中图分类临床医学;
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2. Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry [J] . Anastyuk S.D., Shevchenko N.M., Nazarenko E.L., Carbohydrate research . 2010,第15期

机译：串联MALDI和ESI质谱分析褐藻海带中高度硫酸化的褐藻聚糖的结构
3. A sulphated fucan from the Laminaria abyssalis inhibits the human T cell lymphotropic virus type 1-induced syncytium formation in HeLa cells. [J] . Romanos MT, Andrada Serpa MJ, Mourao PA, Antiviral chemistry & chemotherapy . 2002,第4期

机译：来自海带的硫酸化岩藻聚糖可抑制人T细胞1型淋巴病毒在HeLa细胞中诱导的合胞体形成。
4. Microscopy Techniques and Technologies Used in Ultrastructural Analyses of Poliovirus Replication Complexes Formed During Infection of HeLa Cells [C] . ER Fischer, V Nair, BT Hansen, Microscopy and microanalysis.;Microscopy and microanalysis. . -1

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5. The structural characterization of fucan sulfate from sea urchin by NMR spectroscopy and methylation analysis. [D] . Christ, Marie Dvorak. 1994

机译：海胆中硫酸呋喃岩的结构表征及核磁共振光谱和甲基化分析。
6. The Effect of Sulfated (1→3)-α-L-Fucan from the Brown Alga Saccharina cichorioides Miyabe on Resveratrol-Induced Apoptosis in Colon Carcinoma Cells [O] . Olesia S. Vishchuk, Svetlana P. Ermakova, Tatyana N. Zvyagintseva 2013

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7. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis [O] . Maria T. V. Romanos, Maria J. Andrada-Serpa, Paulo A. S. Mourão, 2011

机译：来自海藻的硫酸化fucan通过HTLV-1游离颗粒抑制HeLa细胞感染：半定量分析

Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semi-quantitative analysis

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